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| Localization and function of microtubule associated protein tau in mouse ovarian granulosa cells |
| Affiliated Hospital of Hubei University of Arts and Sciences College, Xiangyang City Central Hospital, Hubei Province, 441021 |
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Abstract Objective: To investigate the expression and function localization of microtubule associated protein tau in mouse ovarian granulosa cells. Methods: 12 female mice were selected, which included 6 adult mice and 6 juvenile mice, and their ovarian granulosa cells were collected and cultured. The granulosa cells of these mice were cultured with or without FSH, and immunohistochemical staining was used to observe the expression of microtubule associated protein tau in the granulosa cells. Then Tau fusion protein was transfected into the granulosa cells, and the localization of tau in the granulosa cells was observed by fluorescence microscopy. Results: The positive expression of tau protein in the ovarian granulosa cells of the adult was significant higher than that of juvenile mice. Among the adult mouse, the positive expression of tau protein in the granulosa cells cultured with FSH was significant higher than that of cells cultured without FSH (P<0.05). Among the juvenile mouse, the positive expression of tau protein in ovarian granulosa cells of cultured with FSH was significant higher than that of ovarian granulosa cells cultured without FSH (P<0.05). Tau protein of ovarian granulosa cells of adult mice was aggregated in nucleus and was radiated. Conclusion: The microtubule associated protein Tau can be highly expressed in ovarian granulosa cells of adult mouse, and its localization is mainly concentrated in the nucleus. The expression and localization of tau can reflect the formation and morphological changes of microtubules, which affect the proliferation and differentiation of microtubule of granulosa cells.
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