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| The expression of serum MiR-222 and estrogen receptor-α (ERα) of pregnant women with gestational diabetes mellitus and its clinical significance |
| Affiliated Hospital of North China Science and Technology University, Tangshan, Hebei Province, 063000 |
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Abstract Objective:To investigate the expression of serum MiR-222 and estrogen receptor-α (ERα) of pregnant women with gestational diabetes mellitus (GDM), and to analyze its clinical significance. Methods: Collected 100 pregnant women with GDM in affiliated hospital of north China Science and Technology University were selected into research group from August 2014 to August 2016, and another 100 normal pregnant women during the same period were selected into control group. The clinical indexes of all included pregnant women in the two groups were observed. qRT-PCR method was used to detect the expressions of serum miR-222 and ER mRNA, Western blot was used to detect the expression level of ERαprotein. the relationship between the expressions of miR-222 or ERαand the clinical indicators of pregnant women with GDM were compared between the two groups. Pearson method was used for further analyzing the correlation between the expression of miR-222 and the expression level of ERα. And according to the test results of the median value of miR-222 and ERαexpression, pregnant women with GDM were divided into high expression group and low expression group, and the relationships between miR-222 or ERαexpression and the prognosis of the newborns were analyzed. Results: The BMI, FPG and FINS of pregnant women in research group were significant higher than those of pregnant women in control group (P<0.05). Compared with the control group, the expression of serum miR-222 of pregnant women in research group was increased significantly (P<0.05), while the expression of ERαmRNA and the level of ER protein expression were decreased significantly (P<0.05). The expression of miR-222 of pregnant women with GDM was positively correlated with BMI, FPG, FINS or TC (P<0.05), while the expression of ERαmRNA was negatively correlated with FPG (P<0.05). Pearson correlation analysis had showed that the expression of miR-222 was negatively correlated with ER mRNA expression (P<0.05), and the expressions of miR-222 or ERαmRNA were significant correlated with neonatal pathological jaundice (P<0.05). Conclusion: The up-regulation of serum miR-222 expression of pregnant women with GDM can regulate down the expression of ER, and then involve in the occurrence and development of GDM. As for pregnant women with GDM, serum miR-222 expression is negatively correlated with ER mRNA expression, so it can be used as a biological indicator for early diagnosing GDM of pregnant women, and also can be as a biological indicator for evaluating neonatal prognosis.
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