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| Clinical value of detection of Ki67, P16, and INp63gene expression for patients with cervical HPV positive and CIN |
| 1. Xiangyang Traditional Chinese Medicine Hospital Affiliated to Hubei University of traditional Chinese Medicine, Xiangyang, Hubei, 441000; 2. The First People's Hospital of Xiangyang City Affiliated To Hubei Medical and Pharmacy College |
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Abstract Objective: To explore the clinical value of detection of Ki67 and P16 combined with INp63 of tumor suppressor gene P53 gene family for diagnosing cervical intraepithelial neoplasia (CIN) of patients with cervical HPV positive. Methods: A total of 240 patients who had been confirmed in department of obstetrics and gynecology of Xiangyang traditional Chinese medicine hospital from January 1 to December 31 of 2017 were divided into normal cervix group (60 cases), LSIL group (60 cases with CIN I), CIN II group (30 cases), HSIL group (30 cases with CIN III), and early cervical cancer group (60 cases) according to the pathological diagnosed results by HE staining. The expressions of P16, Ki67 and INp63 in cervical tissue of all patients were detected by immunohistochemical. And the sensitivity, specificity, positive predictive value and negative predictive value of different diagnosing method were analyzed. And diagnostic efficacy of the combined detection was evaluated. Results: The expressions of Ki67, P16 and INp63 were correlated with the grade of cervical lesions. The positive expressions rates of Ki67, P16 and INp63 were increased with the increase of grade of CIN (P<0.001). The sensitivity and specificity of P16 combined with Ki67 were 95.6% and 83.4%, respectively. The sensitivity and the specificity of series of P16 and Ki67 were 82.3% and 100.0%, respectively. The sensitivity and specificity of P16 and Ki67 combined with INp63 were 96.7% and 85.2%, respectively. The sensitivity and the specificity of P16 and Ki67 combined with INp63 were 87.1% and 100.0%. Conclusion: The expression of Ki67, P16 and INp63 can reflect the grade of CIN. The detection of P16 and Ki67 combined with INp63 can improve the sensitivity and specificity of diagnosing CIN in clinic practice.
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