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| Clinical values of p16/mcm2 and human papillomavirus detections of patients for screening their cervical lesion |
1.Xuzhou Medical University, Xuzhou, Jiangsu Province, 221004; 2.Xuzhou Cancer Hospital, Xuzhou; 3.The Affiliated Hospital of Xuzhou Medical University, Xuzhou
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Abstract To detect the expression of p16/mcm2 in cervical exfoliated cells of patients with cervical lesions, and to explore the clinical values of p16/mcm2 and human papillomavirus (HPV) detections of patients for screening their cervical lesion. Methods: 1045 women who were screened for cervical cancer in the gynaecology outpatient clinic or ward of Xuzhou cancer hospital and met the included standard from May 2023 to October 2023 were selected as the study subjects. These women were given the p16/mcm2 immunocytochemistry double-staining and HPV detections. The women with the lesions of atypical squamous epithelial cells of unknown significance (ASC-US) or above by p16/mcm2 double-staining diagnosis or with the HPV16/18 positivity were given colposcopy, biopsy and pathological examinations. Results: The positive rate of p16/mcm2 increased in order of the women with HPV negative, of the women with low-risk HPV infection, of the women with the other 12 types of HPV positive and of the women with HPV16/18 positive (P=0.000). The positive expression of p16/mcm2 of the women with the positive other 12 types of HPV was significantly higher than that of the women with the negative HPV (OR=4.28, 95%CI 2.02-9.04). The positive expression of p16/mcm2 of women with positive HPV 16/18 was significantly higher than that of the women with the negative HPV (OR=76.64, 95%CI 36.05-162.93). The positive expression of p16/mcm2 of the women with positive HPV16/18 was significantly higher than that of the women with the positive other 12 types of HPV. The positive expression of the women increased with the severity of their cervical lesions (all P<0.05). The sensitivity of p16/mcm2 detection of the women for predicting their cervical lesions of CINⅠ, CINⅡ and CIN Ⅲ were 85.0%, 89.1% and 94.3%, respectively, and the positive predictive value of p16/mcm2 of the women for predicting their cervical lesions of CINⅠ, CINⅡ and CIN Ⅲ were 94.1%, 97.0% and 97.0%, respectively, and both of which of the women were significantly higher than those of HPV detection. Conclusion: There is a correlation between the positive p16/mcm2 double staining results of the women and their HPV infection, and both can be used for screening the cervical lesions of the women. Compared with that of HPV detection, the p16/mcm2 double staining detection has higher sensitivity and specificity, so the combined detections of p16/mcm2 double staining and HPV of the patients can be as the good application prospect of the cervical cancer screening method.
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