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Abstract Objective: To explore the role of progesterone on the expression of interleukin (IL) 8 on fetal maternal interface in mice with repeated spontaneous abortion. Methods: A classical (CBA/J ×DBA/2) animal model of spontaneous abortion was set up, and the expression of IL-8 protein and mRNA of decidua and villi in spontaneous abortion mice was measured by immunohistochemical staining method and reverse transcription-polymerase chain reaction (RT-PCR). Results: In the progesterone group, the serum concentration of progesterone ［(84.91±10.05) ng/ml］ was significantly higher than that in the model group ［(41.83±9.77) ng/ml］, while the IL-8 level ［(615.23±213.62) pg/ml］ was significantly lower than that in the model group ［(1653.14±321.25) pg/ml, P<0.01］. There were no significant differences in serum IL-8 and progesterone concentrations between the progesterone group and the control group (P all >0.05). The results of immunohistochemical staining and RT-PCR showed that in the chorionic villi, relative contents of IL-8 protein and mRNA (0.067±0.028, 61.28±4.13) in the progesterone group were lower than those in the model group (0.135±0.012, 85.72±2.63), showing significent difference (P<0.01); there were no significant difference in relative contents of IL-8 protein and mRNA in the chorionic villi between progesterone group and the normal group (0.059±0.021, 58.33±2.87, P>0.05). In addition, in the decidual tissue, the relative contents of IL-8 protein and mRNA in the progesterone group were significantly lower than those in the model group (P<0.05), while similar with those of the normal group (P>0.05). Conclusion: Progesterone could down-regulate the expression of IL-8 on fetal maternal interface in spontaneous abortion mice.
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