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Jia Qiuli, Lin Sheng, Duan Fangling, et al
Objective: To explore the association of genetic variations in CNTNAP2 gene with autism in southern Chinese Han population. Methods: Blood samples from 45 patients with autism and their core family members were recruited in this study. The exons and their flanking regions of CNTNAP2 gene were directly sequenced. After aligning the sequence from 45 patients with autism and their core family, DNA variants were identified, including rare mutations, indels and SNPs, and their allele frequency and genotypes were compared with those obtained from the 1000 Genome Project. The transmission of these variations were also studied in the core family. Finally, computational algorithms were employed to estimate deleterious effects of these variations to the protein structure and function. Results: A total of 4 rare mutations, 3 indels and 13 SNPs were identified from patients with autism, including 2 missense mutations p.747 A>V and p.1102 V>I. 3 of these mutations and indels were de novo origination, the other four were inherited from parents. The allele frequency of rs6973990 G>T was diverse between patients with autism and healthy controls (χ2=9.356, P=0.002). Moreover, 5 SNPs were calculated to be significantly different in an effective allele dominant model or recessive model respectively (P<0.05). In addition, computer-assistant analysis indicated that the p.747 A>V mutation might be functionally deleterious. Conclusion: Genetic variations of CNTNAP2 gene are perhaps involved in the pathogenic mechanism of autism in southern Chinese Han population.
2014 Vol. 22 (2): 76- [Abstract]（
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Wu Yan, Liu Shuhong, Dan Zeng, et al
Objective: To explore the effect of quinacrine on testis injury induced by microwave irradiation in mice. Methods: Male mice were randomly divided into four groups, namely normal control group, radiation control group, low-dose of quinacrine group (12.6mg/kg) and high-dose of quinacrine group (50.4mg/kg). Quinacrine was orally given to mice one hour before microwave irradiation, and except normal control group, all animals received 50mW/cm2 microwave irradiation for 30 minutes. Immediately after irradiation, and 1 day, 2 days, 7 days after irradiation, testis injury of mice was detected by the pathophysiologic method, while, pathophysiologic detection for animals of normal control group was finished 7 days after irradiation experiment. Meanwhile, the expression leval of heat shock protein (HSP) 70 in mice testis was analyzed using Western Blot. Results: The pathological examination showed that the microwave irradiation induced seminiferous tubules irregular shape, spermatogenic cells edema and irregular arrangement, as well as interstitial hyperemia. While in quinacrine groups, spermatogenic cells arranged in rows, and suffered less swelling compared with the radiation control group. Moreover, the microwave irradiation resulted in the increased level of HSP70 expression in mice testis, while, quinacrine pretreatment further up-regulated the expression level of HSP70. Conclusion: The protection from testis injury with microwave irradiation by quinacrine may be related to anti-inflammatory and anti-apoptosis effects through the increased level of HSP70 expression.
2014 Vol. 22 (2): 84- [Abstract]（
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Wu Junqing, Wang Kewei, Zhou Ying, et al
Objective: To investigate situations about contraception in married female migrants and to identify potential influential factors, with the aim of providing the critical information for contraceptive-related intervention studies. Methods: A cross-sectional study was adopted in this study, and the data were collected with face-to-face questionnaire interview. Descriptive study was used for the analysis of contraceptive status and crosstab analysis for its influence factors. Results：There were 1691 women (92.5%) who took contraceptives among 1828 married women，and the main contraceptives were condom (38.6%), intrauterine devices (IUD, 33.7%), sterilization (8.6%), oral contraceptives (2.1%) and others (17.0%). Compared to the subjects with the elder age, poorer education background, and lower household income and having children, female migrants with younger age, good education background, high household income, and without childbirth, preferred to use non-permanent contraceptives, such as IUD, condom, oral contraceptives, and so on. Conclusion: There are some factors influencing the contraceptive use, such as age, education, economic status and whether having children. It is necessary to advance IEC program on contraceptive knowledge，sexual behavior intervention，and to strengthen the capacity of informed choice on contraception among female migrants.
2014 Vol. 22 (2): 88- [Abstract]（
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Tu Xiaowen, Lou Chaohua, Sun Feng, et al
Objective: To understand the coping behavior of the victim of husband physical violence among married migrant women of reproductive age. Methods: A total of 103 victims of husband physical violence were interviewed among 961 eligible women who were selected through community-based multiple-stage sampling method in one urban district in Shanghai between April and May 2010. Results: Among victims of husband physical violence, 66.6% of them had ever experienced the moderate violence and 33.4% had ever experienced the severe one. In total, 41.9% of the victim had never fought back physically against their husband and 59.1% had not told anybody about their violence. People who they had ever talked to and would like to get help from were mostly their family and friends. Only a few of them had ever told or would like to get help from formal services such as police/staff of Women's Union or the Neighborhood Committee. Traditional attitude that "Don't wash your dirty linen in public" was the main reason they did not seek professional help. Conclusion: The proportion of women who had ever sought help after physical violence was low. Education on domestic violence among married migrant women of reproductive age is urgently needed to change their attitudes to domestic violence and build their coping skills to reduce the domestic violence.
2014 Vol. 22 (2): 91- [Abstract]（
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Li Li, Yang Weiping, Huang Ying
Objective: To explore the knowledge and behavior of preconception care as well as the demand and intention for preconception care services among newly-married couples so as to provide effective preconception care services and improve the method of preconception care in the economic developed areas. Methods: A total of 1030 newly-married couples in Changning District were recruited by a random method. And a self-designed questionnaire was used to survey the intention and influence factors of preconception care. Results: There was a significant difference in the awareness of related knowledge on preconception care between male respondents and female ones (P<0.05). As for the preconception care, 92.7% of the respondents thought it was necessary; 91.0% supported preconception care. The nutrition before pregnancy(82.9%), the knowledge on genetics and eugenics(80.9%) as well as the essence of reproduction(80.6%) were the top three requirements. About 82.5% of the respondents preferred to reading materials related to preconception care. Conclusion: Newly-married couples consider it necessary to promote the education on the knowledge of preconception care and to proform the preconception examination.
2014 Vol. 22 (2): 95- [Abstract]（
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Zhu Yiling, Tang Li, Yang Xiaohua, et al
Objective: To explore the application value of measuring fetal nuchal translucency and nasal bone in first-trimester screening for Down syndrome (DS). Methods: A total of 1206 pregnant women with 11-13+6 weeks of gestational ages were randomly assigned into group NT and group NT+NB. The nuchal translucency was measured in group NT, and both nuchal translucency and nasal bone were measured in group NT+NB. The amniocentesis was conducted on patients with positive results. The sensitivity, specificity, false positive rate, false negative rate, positive predictive value, negative predictive value and Youden index were evaluated in two groups. Results:The sensitivity of the screening method in NT+NB group and NT group was 100% and 90%, respectively (P<0.05); the specificity in NT+NB group and NT group was 97.45% and 98.15%, respectively; the false positive rate in NT+NB group and NT group was 2.55% and 1.85%, respectively; the false negative rate in NT+NB group and NT group was 0 and 10%, respectively (P<0.05); the positive predictive value in NT+NB group and NT group was 48.25% and 45%, respectively; the negative predictive value in NT+NB group and NT group was 100% and 99.83%, respectively; and the Youden index in NT+NB group and NT group was 97.45% and 88%, respectively (P<0.05). Conclusion: Measurements of Nuchal translucency and nasal bone can significantly improve the sensitivity and correct index as well as reduce the rate of misdiagnosis in DS screening.
2014 Vol. 22 (2): 98- [Abstract]（
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Gao Wenxiao, Cui Shihong, Li Genxia, et al
Objective: To explore the correlation between preconception body mass index (BMI), weight gained during pregnancy and serum glucose and lipid levels of late pregnancy retrospectively. Methods: Fifty pregnancy women with gestational diabetes mellitus (GDM), 50 with pregnancy hypertension disease (PHD), 30 with gestational diabetes mellitus (GDM) complicated with PHD and 50 women with a normal pregnancy (control group) who were singleton and at late pregnancy in Third Affiliated Hospital of Zhengzhou University during January to June 2013 were recruited. The first three groups served as the metabolic disorder group. The BMI, weight gained during pregnancy, concentrations of fasting plasma glucose (FPG), triglyceride (TG), total cholesterol (TC), high density lipoprotein (HDL), low density lipoprotein (LDL) and blood pressure level were evaluated. Results: The preconception BMI, weight gained during pregnancy as well as FPG and TG levels of the metabolic disorder group were significantly higher than those of the control group (P<0.05). Preconception BMI was positively correlated with levels of systolic pressure and diastolic pressure respectively (r=0.256, P<0.05; r=0.239, P<0.05); The weight gained during pregnancy was positively correlated with TG and FPG levels respectively (r=0.340, P<0.05; r=0.221, P<0.05), but negatively correlated with HDL level (r=-0.331, P<0.05). Conclusion: The preconception BMI, weight gained during pregnancy are closely related to metabolic indicators, and may play important roles in the pathogenesis of metabolic disorders. Strictly taking control of preconception weight and nutrition intake during pregnancy might help to prevent gestational metabolic disorders.
2014 Vol. 22 (2): 102- [Abstract]（
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Xu Jinjing, Wu Yuning
Objective: To investigate effects of Tianjing Tongluo Recipe combined with estrogen on endometrial thickness and appearance. Methods: Forty-five women with persistent thin endometrium who had received high-doses of estrogen and were waiting for in vitro fertilization and embryo transfer (IVF-ET) were recruited. Women with syndrome of kidney deficiency and blood stasis were given Tianjing Tongluo Recipe and estrogen for 6 menstrual cycles. The changes of endometrial thickness and pattern were compared before and after treatment, and the pregnancy rate was evaluated 6 months after the treatment by follow-up survey. Results: Endometrial thickness and appearance at the follicle maturation after the treatment were significantly better, and the endometrial improvement rate was 82.22%. The pregnancy rate was 68.89% in six months' follow-ups. Conclusion: Tianjing Tongluo Recipe combined with estrogen has a remarkable curative effect in the treatment of thin endometrium with estrogen hyporesponsiveness.
2014 Vol. 22 (2): 106- [Abstract]（
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Hao Yingying, Chen Guilan, Zhou Jiawen, et al
Objective: To explore the clinical effect and side effects of YOUMET cervical dilating rod used for cervical orifice dilation before IUD insertion and removal and artificial abortion operations. Methods: A total of 275 women seeking for cervical dilation during IUD insertion and removal operations, and artificial abortion-vacuum aspiration operation for pregnancy within 10 weeks were randomly divided into two groups. There were 137 women in the observation group and YOUMET cervical dilating rod was used, while 138 served as the control and Gongshuning glue stick was used. The cervical softening and dilatation status, analgesic effect, and comprehensive reactions during operation were observed. Women were followed up since the 20th day after operation. Results: For the two groups, there was no statistical significance in general biological characteristics (P>0.05). Dilating effects in IUD inserting and removing operations during child-bearing period were 100% and 96%, which showed no statistical significance. Dilating effects in artificial abortion operation and IUD removing operation during menopause showed statistical significance (P<0.05). And incidences of side effect, which was pain during insertion, were 4% and 25% in turns, showing significant difference (P<0.05). Incidences of pain during indwelling period for both groups were comparatively low, which showed no significant difference. There was no adverse record related to the usage of cervical orifice dilating products in post-operation follow-up visits (P>0.05). Conclusion: YOUMET cervical dilating rod has trustworthy and safe dilating effect. It is applicable to cervical dilation before IUD insertion and removal operations, and vacuum aspiration operation for termination of pregnancy within 10 weeks.
2014 Vol. 22 (2): 109- [Abstract]（
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Li Jianwei
Objective: To explore effects of family planning operations on mental health status and quality of life. Methods: A total of 396 women seeking for family planning operations were recruited and divided into two groups: IUD group (n=232) and artificial abortion group (n=164). Two hundred women experiencing non-gynecologic operations served as the control. The symptom checklist (SCL-90) and the 36-item short-form health survey (SF-36) were used to evaluate the mental health status and quality of life . Results: Except interpersonal sensitivity and paranoid ideation, scores of SCL-90 in artificial abortion group were higher than that of the IUD group (P<0.05), while the latter had no significant difference when compared with the control group (P>0.05). Scores of role emotional and mental health in the artificial abortion group were lower than those of the control group (P<0.05), while there were no significant differences in the score for each symptom between the IUD goup and the control group (P>0.05). Conclusion: IUD insertion (or removal) and artificial abortion might affect both psychological status and quality of life for women, but smaller is found in insertion or removal of IUD.
2014 Vol. 22 (2): 113- [Abstract]（
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