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Hou Zihong,He Shuang,Gu Xiangying,Zhang Aijuan,Yue Xiuying
To explore the pathological change and expressions of Survivin and caspase-3 in villi in women with different gestational ages. Methods: Sixty specimens were collected from women seeking for termination of early pregnancy in Department of Gynecology and Obstetrics of The General Hospital of Tianjin Medical University between May 1, 2011 and June 30, 2011. Thirty women with 40 to 49 days of gestational ages were randomly divided into two groups (control group A and study group A), and the other 30 women with 50 to 63 days of gestational ages were randomly divided into two groups (control group B and study group B). Women in the study group were treated with 150 microgram mifepristone before artificial abortion with suction evacuation. The villus tissue was collected. The pathological change of villus was evaluated with a light microscope and expression levels of Survivin and caspase-3 in villi were detected by immunohistochemistry technique. Results: In study groups, proliferation nodules of trophoblastic cells were significantly reduced (P<0.05). There were no significant differences in the number of proliferation nodules of trophoblastic cells between control A and B groups as well as study A and B groups (P both >0.05). Expressions of Survivin were observed in the nucleus and cytoplasm of the villi in the control group and the study group. The expression level of Survivin was lower in the study group (P<0.05). There were no significant differences in expression level of Survivin between control A and B groups as well as study A and B groups (P both >0.05). Expressions of caspase-3 were observed in the cytoplasm of the villi in the control group and the study group. The expression level of caspase-3 was lower in the control group (P<0.05). There were no significant differences in expression level of caspase-3 between control A and B groups as well as study A and B groups (P both >0.05). Conclusion: Mifepristone could inhibit the formation of proliferation nodules of trophoblastic cells. And the mifepristone could inhibit the expression of Survivin and improve that of caspase-3, resulting in termination of early pregnancy due to the promotion of apoptosis. It can be suggested that there is the same effect of 150 microgram mifepristone on medical abortion for different gestational ages of early pregnaney.
2013 Vol. 21 (9): 580-584 [Abstract]（
59
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Yin Qiaozhi,Lu Hua,Li Limin
To explore the mechanism of increasing the number of vessel in vaginal lamina propria of ovariectomized rats by Zuogui Pill and Yougui Pill. Methods: Female mature rats were randomly divided into 6 groups: normal group, sham-operated group, model group, Premarin group, Zuogui Pill and Yougui Pill group. Rats in the normal group, sham-operated group, model group were fed with saline, and those in Premarin group were fed with Premarin (62.5ug/100g). Rats of Zuogui Pill and Yougui Pill group were fed in the 1-5 formula respectively in dosage of 15 times of adults. All rats were fed for 12 weeks. The thickness of vaginal mucosa, covering epithelium, vaginal folds and vessel numbers of lamina propria were measured by Mias-2000 Image Analysis System. The expression levels of VEGF and VEGFR-1 in the uterus and vagina were evaluated by immunohistochemistry and fluorescent quantitation PCR. Results: Compared with sham-operated and normal groups, thinner vaginal mucosa, less vaginal folds and blood vessels in lamina propria were found in the model group (P<0.05). Compared with the model group, thicker vaginal mucosa, more vaginal folds and blood vessels in lamina propria were found in Premarin group (P<0.05), and more blood vessels in lamina propria were found in Zuogui Pill and Yougui Pill group (P<0.05). Compared with sham-operated and normal groups, expression levels of vaginal VEGF and VEGFR-1 were significantly lower in the model group (P<0.05). Compared with the model group, expression levels of vaginal VEGF基金项目:国家自然基金(30873279);国家重点基础研究发展计划(973计划)(2010CB530403)收稿日期:2013-05-15 修回日期:2013-05-23*通讯作者:kjclh@126.comand VEGFR-1 were significantly higher in Premarin group (P<0.05). Higher vaginal VEGF expression level was found in Zuogui Pill and Yougui Pill group (P<0.05), and no difference in VEGFR-1 expression level among between the model group, Zuogui Pill group and Yougui Pill group (P>0.05). Concentrations of vaginal VEGF mRNA in sham-operated, normal, Premarin, Zuogui Pill and Yougui Pill groups were 5.21, 3.54, 3.20, 6.60,5.92 times of the model group, respectively. And concentrations of vaginal VEGFR-1 mRNA in sham-operated, normal, Premarin, Zuogui Pill and Yougui Pill groups were 1.39, 1.47, 1.34, 1.19, 1.02 times of the model group, respectively. Conclusion: Zuogui Pill and Yougui Pill might improve the number of blood vessel in vaginal lamina propria by regulating vaginal VEGF and its mRNA in ovariectomized rats.
2013 Vol. 21 (9): 585-590 [Abstract]（
63
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Xi Maomao,Li Yan,Zhang Lifeng,Ji Ning,Zhou You,Cheng Yimin
To explore factors of promoting the female condom (FC) among female sex workers (FSWs) in China. Methods: The field study was conducted in Enping City between September and December 2007. The FSWs′ knowledge and usage of FC were investigated in this study. Results: There were 291 women in the final analysis. About 89.7% of respondents used contraceptive measures, and 65.6% used male condoms. About 16.2% of respondents were diagnosed with a sexually transmitted disease. After using FC, the median score of FC knowledge among respondents improved significantly (t=14.48, P＜0.01). After usage, 64.6% were satisfied and 64.9% wanted to continue to use. The multi-factor analysis of continuing to use showed risk factors include less discomfort (OR = 2.28) and interference for sex (OR =3.65), more advantages (OR=2.33), and no objection from male partner (OR = 2.50). Conclusion: To promote the domestic FC among the sex workers in China, measures should be taken to strengthen propaganda on FC advantages and knowledge both in men and women, and training on usage of FC to reduce discomfort in sex, interference for sex and the probability of the objection from male partners.
2013 Vol. 21 (9): 595-598 [Abstract]（
49
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Wang Qiling,Zeng Meizhen,Zheng Ruihua,Tang Lixin,Huang Jiangtao,Xu Shanshan,Chen Rong,Li Jianwen,Lin Liang
To explore the knowledge, attitude and practice (KAP) of newly married childbearing women toward the free preconception health check for eugenic to provide scientific basis for primary prevention of birth defect. Methods: A total of 1178 newly married childbearing women were surveyed on their KAP related to free preconception health check for eugenic in Wuchuan County and Huicheng District between September 2011 and August 2012. Results: The respondents were 25.19±3.34 years old and 71.39% of them were planning to get a pregnancy. About 83.4% knew the prevention of birth defects would begin before pregnancy and 76.4% knew birth defects were caused by both genetic and environmental factors. About 86.76% of them were willing to have a free preconception health check for eugenic. The respondents knew more about基金项目:广东省计划生育科技项目“孕前优生检查群众依从性研究”(20110201); 广东省科技项目“惠州地区人口出生缺陷一级预防干预策略与评估研究”(2012B032000002) 收稿日期:2013-03-18 修回日期:2013-06-05*通讯作者:tang-lixin@126.comthe clinical laboratory tests and physical examination and less about screening for thalassemia, glucose-6-phosphate dehydrogenase deficiency and other genetic diseases as well as free counsel, health education and risk assessment. The free eugenics check was desired for those planning to get pregnant or already being pregnant or their friends had been participating in similar free checks. The factors, such as the reliability and confidentiality of the results of free checks and the addition of the risk assessment and other measures, could affect the respondents′ willingness to participate in a free preconception health check for eugenic. Conclusion: It should be promoted for the scientific propaganda on knowledge of birth defects and preconception care in the primary prevention of birth defect. The participate rate would be increased if the prevention service is provided in line with the requirement and the technical services are improved in the family planning technical service institutions.
2013 Vol. 21 (9): 599-602 [Abstract]（
61
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Xin Limei,Zhong Chunli,Zhang Weixian,Ren Xiaoling,Wang Dan
To explore clinical effects and safety of MYCu intrauterine device (IUD) and MCuⅡfunctional IUD (MCuⅡIUD). Methods: A total of 400 women seeking for IUD insertion for contraception in the outpatient department were divided into MYCu IUD group (n=200) and MCuⅡIUD group (n=200) with a random envelope method and followed up at the 1st, 3rd, 6th, 12nd, and 24th month after insertion. Results: Twenty-four months after insertion, 90.5/100 women year and 88.5/100 women year in MYCu IUD group and MCuⅡIUD group persisted on use, respectively. Rates of pregnancy with IUD in situ were 1.01/100 women year and 1.56/100 women year, respectively. Expulsion rates were 1.05/100 women year and 1.04/100 women year, respectively. And discontinuation rates due to bleeding/pain were 2.06/100 women year and 2.59/100 women year, respectively. There were no significant differences in indicators mentioned above between two groups. One month and 3 months after insertion, the occurrence rate of adverse effects (mainly abnormal menstruation and pain) of MYCu IUD group was significantly lower than that of MCuⅡIUD group (P<0.05). Conclusion: MYCu IUD and MCuⅡIUD have the characteristics of better contraceptive effect, lower expulsion rate and less adverse effects. Indomethacin-containing MYCu IUD is a comparative ideal IUD because it can reduce eht incidence of adverse effects during early insertion.
2013 Vol. 21 (9): 603-605 [Abstract]（
64
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Li Kejun,Zhang Huiying,Zhang Yanfang,Han Yukun
To explore the expression and clinical significance of a tumor suppressor gene, PTEN, in endometrium of polycystic ovary syndrome (PCOS) patients. Methods: PTEN of endometrial specimen were detected with immuno histochemistry in 36 PCOS patients (PCOS group) and 28 women without PCOS (control group). Six kinds of serum sex hormone, fasting plasma glucose and insulin were also detected in the PCOS group, and pathological examination was conducted as well. Women in PCOS group were redivided into endometrial lesion group and normal endometrium group according to the pathological result, insulin resistance group and no insulin resistance group, and overweight and obesity group and normal weight group according to body mass index. Results: The expression level of PTEN in endometrium of PCOS group was significantly lower than that in the control group (P＜0.05). The expression level of PTEN in endometrial lesion group was significantly lower than those in normal endometrium group (P＜0.05)and the control group (P<0.001). Between the insulin resistance group and no insulin resistance group, there was no significant difference in the expression level of PTEN. The expression level of PTEN in insulin resistance group were significantly lower than that in the control group (P＜0.05). There was no significant difference in the expression level of PTEN between no insulin resistance group and the control group. The expression level of PTEN in PCOS overweight and obesity group was significantly lower than that in normal weight group (P<0.01) and the control group (P<0.001). Between the normal weight group and the control group, there was no significant difference in the expression level of PTEN. The negative rate of PTEN expression in endometrial lesion group was significantly lower than that in normal endometrial group and the control group (P all<0.01). There was no significant difference in the negative rate of PTEN expression between normal endometrial group of PCOS and the control group(P>0.05). The decline or negative expression of PTEN had correlations with endometrial lesions and insulin resistance. Conclusion: The detection of the expression of PTEN in endometrium of PCOS patients could be used as a clinical biological indicator to predict endometrial hyperplasia and cancer.
2013 Vol. 21 (9): 607-610 [Abstract]（
59
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Shen Shuna,Lu Shengqun,Wang Hui
To explore the function of L-Carnitine in pathogenesis of polycystic ovary syndrome (PCOS). Methods: Thirty women seeking for PCOS treatment and 30 infertility women for male factors or abnormal oviduct served as the study group and the control group, respectively. The level of serum L-Carnitine was tested by high performance liquid chromatography. And the levels of serum leptin and hormone were evaluated by ELISA and chemical luminescence method, respectively. The correlations of parameters mentioned above were analyzed. Results: The levels of L-Carnitine, leptin, luteinizing Hormone (LH), the ratio of LH to follicle-stimulating hormone (FSH) and testosterone (T) in PCOS patients were significantly higher than those of the control group. There were negative correlations between the level of L-Carnitine and the levels of T (r=-0.421, P<0.05) and leptin (r=-0.526, P<0.05), respectively. Conclusion: L-Carnitine may play an important role in occurrence and development of PCOS.
2013 Vol. 21 (9): 611-613 [Abstract]（
61
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Zhu Dong
To investigate the clinical effect of estrogen and progesterone on vaginal bleeding after medical abortion and relationships between abortion-related outcomes and levels of progesterone (P) and estradiol (E2) . Methods: A total of 85 women seeking for the routine medical abortion were randomly divided into recurrent short-acting oral contraceptives (COC) group (n=30), estrogen and progesterone combination group (E + P) group (n=28), hemostatic agent group (control group, n=27). The amount of vaginal bleeding, the duration of vaginal bleeding and relationships between abortion-related outcomes and levels of P and E2 were evaluated. Results: The amount of vaginal bleeding of E + P group was lower than those of COC group and the control group, and both the duration of the vaginal bleeding and the interval between medical abortion and menstruation retrieve of E + P group were shorter than those of latter two groups (P all <0.05). While there were no significant differences in the amount of vaginal bleeding, the duration of vaginal bleeding, and the interval between medical abortion and menstruation retrieve between COC group and the control group (P>0.05). The complete abortion rate of E + P group (100.0%) and COC group (90.0%) were significantly higher than that of the control group (77.78%). After the treatment, the E2 level of the complete abortion group on the 14th day was significantly higher than that on the 7th day (P <0.05), and the P level on the 14th day was significantly lower than that on the 21st day (P<0.05). In the incomplete group E2 levels at different times were not significantly different (P> 0.05), while the P level on the 14th day after the treatment was significantly lower than that on the 7th day (P<0.05). Conclusion: In the medical abortion, the decline of estrogen and progesterone levels may be one of important factors of continuous vaginal bleeding after abortion. The higher rate of the complete abortion as well as the shorter duration and the less amount of of vaginal bleeding could be achieved by increasing levels of E2 and P in the medical abortion.
2013 Vol. 21 (9): 614-617 [Abstract]（
49
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Lv Yonghuan,Song Xueru,Lu Linlin,Zhao Xiaohui,Lv Rui,Bai Xiaohong
To explore the application of array comparative genomic hybridization (aCGH) in screening aneuploidy of human embryos before transfer. Methods: Four cryopreserved normal 8-cell phrase embryos were selected from women who underwent in vitro fertilization treatment in Reproductive Medicine Center of Tianjin Medical University General Hospital. Couples voluntarily donated frozen embryos for research and signed informed consent. Blastomeres were obtained from four frozen-thawed embryos, then multiple displacement amplification was conducted. After enzyme digestion, fluorescent labeling, purification and quantify, amplification products hybridized using aCGH chip. Hybridized aCGH chip was scanned by Agilent Scanner. Obtained image was read and analyzed with Feature exaction 9.5. Results: Samples 1-4 were obtained from embryo 1. And the karyotype of sample 1 detecting by aCGH was 47,XX,del(5) (p15.33-p12), del(9) (q13-q34.13), +21. It was different from sample 2-4 which were 46,XX. Both of sample 5 and 6 were obtained from embryo 2. And the karyotypes of them were 47,XX,+19. Sample 7 and 8 were obtained from embryo 3, both of them were 46,XX. Samples 9 and 10 were obtained from embryo 4, and their karyotypes were 46,XY. Conclusion: aCGH can be successfully used to detect whole genome aneuploidy of human embryos before transfer.
2013 Vol. 21 (9): 618-622 [Abstract]（
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Xie Lichun,DuanShan,Zhong Yuling,Huang Yin
To explore the recurrence of autism in families with two children to provide the scientific basis for birth defect prevention. Methods: Families of children with autism diagnosed in medical authentication between 1990 and 2010 were followed-up through letters or telephone. The development and health status of the second chilel were evaluated. Results: The incidence of autism was increased year by year in the medical authentication. The follow-up rate was 97.16%, and 68.42% of families in this survey have their second children. The sex ratio was 1.45. There were 6 children with autism and 6 children were suspicious with autism. The positive rate was 10%, and the sex ratio was 2.0 (8:4). The recurrent rates of autism were 11.27% and 8.16% in boys and girls, respectively (χ2=0.310, P=0.577). Conclusion: Compared with the normal group and other disabled children, the recurrence rate of autism in the second child is significantly higher in families with autistic children. Genetic screening and consultation need to be promoted in families applying for medical authentication due to autism.
2013 Vol. 21 (9): 659-666 [Abstract]（
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