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Niu Ziru,Yue Xiaojing,Kong Qunyu,Wang Yuanfen,Hou Caiying,Yao Yuanqing
To determine whether embryo culture at low oxygen concentrations could improve clinical outcome in assisted reproductive technology. Methods: Prospective, randomised, controlled studies of application of low oxygen concentrations in embryo culture were searched and screened in PubMed, Ebase, Cochrane Library, China National Knowledge Internet(CNKI), Chinese Biological Medicine Disk(CBM) and Database of Wanfang. After quality assessment and data extraction, a meta analysis was conducted in implantation rates and pregnancy rates by Revman5.1 software.Results: After exclusion and selection, ten studies with a total of 5520 cases (2645 with low oxygen and 2875 with ambient oxygen) were included in the analysis. Evidence of a beneficial effect of culturing in low oxygen concentration was found for higher implantation rate (OR:1.16, 95%CI:1.05-1.29, P=0.01) , ongoing pregnancy rate (OR:1.32, 95%CI:1.12-1.55, P<0.001) and clinical pregnancy rate (OR:1.28, 95%CI:1.07-1.53, P=0.008). There were no evidence that culturing embryos under low oxygen concentrations resulted in higher numbers of adverse events such as multiple pregnancies (OR:1.19, 95%CI: 0.67-2.14, P=0.32) or miscarriages (OR:0.98, 95%CI:0.63-1.53, P=0.93). Conclusion: The results of this meta-analysis suggest that culturing embryos under conditions with low oxygen concentrations could improve the success rate of assisted reproductive technology, resulting in high pregnancy rate without increasing the risk of adverse events. Further reseaeches and more systematic reviews are needed.
2013 Vol. 21 (5): 295-301 [Abstract]（
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Li Juan,Zhang Yanping,Zhang Lihong ,Gai Ling,Qiu Yi,Wei Bin,Wang Hongyan,Wu Aihua,Wang Leiguang
To develop embryonic stem cell lines derived from blastocyst from frozen human blastomere with the sequential co-culture method. Mehtods: Thawed human blastomeres were cultured in a single layer cumulus cell sequential co-culture system. Assisted hatching was conducted when blastocysts were developed. The hatched blastocysts were then cultured with mouse embryonic fibroblast (MEF) feeder layer cell for 6 days. And then a conditional culture medium including 20% MEF was used until the primary clone was developed. The growth status of human embryonic stem cell colony differentiation and the biology identification were confirmed with alkaline phosphatase staining, detection of Oct-4 gene expression, karyotype analysis and in vitro differentiation experiments. Results: Nine frozen human blastomere cultured with a sequential co-culture method, and six of them developed to blastocysts. And ultimately, one of them developed to a embryonic stem cell line. Characteristics identified in this cell line were as follow: showing alkaline phosphatase activity, expressing Oct-4 gene which was the cell-specific marker of embryonic stem cell, forming embryoid bodies, developing to the automatic rhythm myocardial cells in vitro and with a karyotype of 46, XY. Conclusion: The sequential co-culture system can improve embryo developmental potential of thawed-frozen embryos and contribute to the establishment of human embryonic stem cell lines.
2013 Vol. 21 (5): 302-305 [Abstract]（
52
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Cui Fengyun,Diao Ying
To investigate the influence of ginkgolide B upon mice embryo implantation.Methods:Kunming female mice without producing,on the 2nd day of pregnancy, were given ginkgolide B in different doses in one side of the uterine horn (2mg/kg, 4 mg/kg, 6 mg/kg, 8 mg/kg, 10 mg/kg) as the experimental side, while equal amount of physiological saline was injected into the uterine horn of the other side as the control side. On the 8th day of pregnancy, the mice were executed for counting the number of embryos. Results: The number of embryos of the test side was a little bit less than that of the control side in the 2mg/kg group (P＞0.05), but less than those of the control sides in the 4 mg/kg and the 6 mg/kg groups (P＜0.05), and very significantly less than those of the control sides in the 8 mg/kg and the 10 mg/kg groups(P＜0.001). In 5 different doses groups of the experimental sides, the number of embryos of the 2mg/kg group was much more than those of the other 4 groups (P<0.05, P<0.001, respectively). And compared with the 8 mg/kg and the 10 mg/kg groups, the number of embryos of the 4 mg/kg and the 6 mg/kg groups were much move (P all＜0.001), though there were no differences between the 4 mg/kg and the 6 mg/kg, the 8 mg/kg and the 10 mg/kg group. Conclusion: Ginkgolide B can inhibit the embryo implantation of the mice, and the effect is correlated with the doses.
2013 Vol. 21 (5): 306-309 [Abstract]（
52
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Liang Hong,Li Li,Chen Xingbao,Wu Shangchun,Miao Maohua,Yuan Wei
To compare the effects of GT300 intrauterine device (IUD) , compound megestrol acetate injection (Injection), and Norplant implant (Norplant) on the quality of life among chinese users at reproductive age. Methods: A multicenter clinical trial was conducted in 25 county-level family planning clinics in 8 provinces. The women who were seeking for contraceptives mentioned above were enrolled. The chinese version of the 36-items short form health survey (SF-36) was used to evaluate the quality of life before and 3 months after contraceptive use. Results: A total of 1356 women at reproductive age were enrolled in 20 clinics, among whom 446 women used IUD, 610 used Injection and 300 used Norplant. The total score of SF-36 slightly improved 3 months after both IUD insertion and Injection use, while it showed a slight decrease 3 months after Norplant use. After adjusting the baseline score, residence, occupation, income, parity, and contraceptive methods used last time, the improvements in the total score 3 months after both Norplant and Injection use were less than that in IUD users. Conclusion: No significant change on quality of life is found before and after IUD, Injection or Norplant use, while the quality of life among IUD users improves.
2013 Vol. 21 (5): 310-315 [Abstract]（
64
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Ying Yanyang,Lin Wenlong,Zhu Renyong,Li Jingrong,Wang Jing
To evaluate the initiative of childbearing-age floating men involving in reproductive health by surveys and interviews. Methods: Through multi-stage randomization, a total of 2 432 childbearing-age men who immigrated from other provinces were enrolled in this study. Surveys were performed and interviews were made among these people. Results: Participants had a relatively low educational level, with the majority graduated from primary and junior middle school (72.3%). The availability of family planning / reproductive health services was lacking. The initiative involvement was insufficient. Conclusion: In order to promote the level of reproductive health, efforts need to be made including enhancing education on reproductive health, improving the awareness of reproductive health, as well as promoting participation in family planning program among male population.
2013 Vol. 21 (5): 316-319 [Abstract]（
51
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Du Hua,Liu Chunyu,He Yan,Liu Xiangtong,Dong Jing,He Dian
To explore the prevalence of postpartum depression (PPD) and related risk factors. Methods: Three hundred pregnant women were evaluated for postnatal health status with Edinburgh postnatal depression scale (EPDS). SPSS18.0 software was used in statistical analysis. Results: With 10 points as the EPDS scale criteria, the prevalence of PPD was 24.33%. Factors influenced the prevalence of PPD were as follow: "the relationship between mother-in-law and daughter-in-law" (OR=2.47, 95%CI=1.12-5.44), "women' s health status before pregnant" (OR=4.99, 95%CI=1.56-15.93) and "the effects of pregnancy on work and income"(OR=1.79, 95%CI=1.02-3.12). Conclusion: The prevalence of PPD is high due to many risk factors. In order to reduce the incidence, more family and psychological supports are necessary.
2013 Vol. 21 (5): 321-323 [Abstract]（
65
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Hao Yingying,Mu Jun,Xu Lujia,Wei Saimei,Chen Yubo,Chai Dongning,Chen Guilan
To explore the clinical effect of MYCu intrauterine device (IUD). Methods: A total of 5200 women were conducted MYCu IUD insertion in 49 centers of 8 provinces selected in different areas of China and follow-up visits 1, 3, 6, and 12 months after insertion. Results: Data of 4998 women finishing 12 months' follow-up visit included in the statistical analysis. The cumulative rate of pregnancy with IUD in situ was 0.29/100 women per year, the cumulative expulsion rate was 0.24/100 women per year, the cumulative discontinuation rate due to bleeding/pain was 1.56/100 women per year, and the cumulative discontinuation rate related to IUD use was 2.08/100 women per year. The cumulative discontinuation rates related to IUD use in 8 provinces were between 3.51/100 women per year and 1.00/100 women per year, showing no statistical differences (P all >0.05). Abnormal menstruation, irregular uterus bleeding, pain in waist and abdomen, and leucorrhea increasing were common after insertion, which gradually alleviated along with the insertion time. Conclusion: With reliable contraceptive effect, low expulsion rate, and low occurrence rate of side effects, MYCuIUD is a comparably ideal IUD.
2013 Vol. 21 (5): 324-327 [Abstract]（
50
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Zuo Huanrong
To evaluate the effects of the extent of laparoscopic adhesiolysis on the pregnancy rate in infertile women with severe adhesions. Methods: A total of 328 women suffering from distal tubal obstruction due to severe pelvic adhesions were randomly divided into the treatment and the control groups. In the treatment group, a limited elective laparoscopic adhesiolysis was performed, in which the working space focused on the fimbria of oviduct and ovary. While in the control group, a thoroughly laparoscopic adhesiolysis was performed in order to restore anatomic structures as near normal as possible. Results: There was no statistically significant difference in the pregnancy rate between the treatment group and the control group. But the incidence of ectopic pregnancy in the treatment group was significant lower than that of the control group. Conclusion: Laparoscopic adhesiolysis may be an effective treatment in female infertility associated with severe adhension. The area of adhesiolysis should be focused on fimbrial and ovarian areas.
2013 Vol. 21 (5): 328-330 [Abstract]（
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Cui Rong,Miao Zhulin,Zhao Wenzhong,Qin Weibin,Wang Xiaolan,Yang Ning,Wang Yifeng
To investigate the effect of initiative immunotheraphy with lymphocytes and low-dose intravenous immunoglobulin (IVIG) in women suffering from unexplained recurrent spontaneous abortion. Methods: Seventy-two patients diagnosed as unexplained recurrent spontaneous abortion were divided into two groups: the study group (n=47, in which women received initiative immunotherapy with lymphocytes and low-dose IVIG) and the control group (n=25, in which women received initiative immunotherapy only with lymphocytes). The pregnancy outcome was evaluated. Results: The pregnancy rate was 89.36% in the study group and only 68.00% in the control group, showing a significant difference between the two groups (P<0.05) . Conclusion: Initiative immunotherapy with lymphocytes and low-dose IVIG may act as an effective and safe method for treatment of unexplained recurrent spontaneous abortion.
2013 Vol. 21 (5): 331-333 [Abstract]（
28
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Zhou Jun,Su Fangming
To compare an amniotic fluid index (AFI) less than 5 cm to an AFI less than the fifth percentile in predicting adverse perinatal outcomes. Methods: A retrospective cohort study was conducted on clinical data of 18 262 pregnant women delivering between 1998 and 2008. Pregnant women with an AFI less than 5 cm and those with an AFI less than the fifth percentile were compared to pregnant women with a normal AFI. The pregnancy outcome such as the number of neonatal intensive care unit (NICU) admission and premature as well as and birth weight were evaluated. Results: There were 145 NICU admissions in pregnant women with an AFI less than 5 cm (RR: 2.2) and 235 in pregnant women with an AFI less than the fifth percentile for gestational age (RR: 2.37). The sensitivity and specificity for NICU admission using an AFI less than 5 cm were 10.9% (95% CI: 9.3%~12.7%) and 95.2% (95% CI:94.9%~95.5%), and those were 17.6% (95% CI: 15.6%~19.8%) and 92.5% (95% CI:92.1%~92.9%) for an AFI less than the fifth percentile (P<0.001). There were statistical differences in the gestational age, AFI, the number of premature and birth weight when comparing an AFI less than 5 cm and that less than the fifth percentile with the normal AFI. Conclusion: Oligohydramnios defined as an AFI less than the fifth percentile would be better for predicting adverse perinatal outcomes.
2013 Vol. 21 (5): 334-336 [Abstract]（
54
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Cao Mingman,Xing Hui
To explore the fluctuating blood glucose in women with gestational diabetes mellitus (GDM) and those with pregestational diabetes mellitus (PGDM) by the continuous glucose monitoring system (CGMS) in order to provide the basis for clinical treatment. Methods: Sixty-three women with GDM and sixty-five ones with PGDM underwent the continuous glucose monitoring. Data derived from the CGMS for 72 hours were evaluated. Results: Fasting blood glucose (FBG) level and fluctuation coefficient in PGDM group were much higher than those of GDM group. There were less hypoglycemia and much more time was needed for peak blood glucose after meal in GDM group. Conclusion: There are still differences in blood glucose profiles of the CGMS for 72h between the two groups though the changes of the FBG level and the concentration of HbA1c are consistent. The fluctuating blood glucose level is recommended for fine regulating the glucose metabolism and personalized medication.
2013 Vol. 21 (5): 337-339 [Abstract]（
50
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Geng Zhen
To explore the value of the enzyme-linked immune response accelerometer in detecting cytomegalovirus immunoglobulin G (CMV IgG). Methods: The CMV IgG measurement was conducted with enzyme-linked immunosorbent assay (the traditional method) and enzyme-linked immune response accelerometer testing (the accelerating method) respectively using standard products, negative and positive control serum and clinical serum samples with known results. The repeatability, sensitivity and specificity of the two methods for detection of CMV IgG were compared. Results: The quantitative result of linear correlation analysis showed the accelerating method and the traditional method were positively correlated. For the accelerating method, the variation coefficient, sensitivity and specificity were 10%-2.1%, 98.2% and 96.3%, respectively, which were all in the prescribed scope. Conclusion: Enzyme-linked immune response accelerometer can be used for the detection of CMV IgG in the clinical practice.
2013 Vol. 21 (5): 340-342 [Abstract]（
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