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Xin Xiaona,Wang Yuanyuan,Ma Liguang,Zhang Ya,Peng Zuoqi,Ma Xu
To explore a new model of preconception care based on information technology and means to improve management and service levels of family planning implement. Methods: The information technology was used in the field of primary prevention of birth defects and solutions were provided for problems in the traditional preconception care services model. A uniform and normative information working platform was established for the informationize service pattern based on electronic reproductive health file in accordance with the current situation and characteristics of population in china. Results: The massive family archives management was regulated. The technical level and working efficiency were improved and service clients increased. Conclusion: Application of the information technology could make it impossible to implement scientific assessment of reproductive health status and risks of reproductive population and promote the birth defect prevention work, resulting in the improvement of the informatization construction of population and family planning services in China.
2013 Vol. 21 (4): 227-230 [Abstract]（
39
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Mo Yi,Liang Fangfang,Chen Yuefeng,Jiang Rulan,Ye Jian,Xie Danni
To construct the shRNA eukaryotic expression plasmid of Follistatin (FS) gene and preliminarily identify its interference efficiency. Methods: According to the FS mRNA nucleotide sequence provided in GENEBANK database, the FS gene shRNA interference target sequence was selected by using the Ambion wed siRNA design software, then cloned into the linearized pLKO.1 plasmid vector to construct the pLKO.1-FS-shRNA recombinant plasmids. After restriction enzyme digestion and sequencing identification, the recombinant plasmids were transiently transfected into 3AO human ovarian cancer cell lines by lipofectamineTM 2000 method, then gene interference efficiency were identified by RT-PCR method. Results: The recombinant shRNA sequence was confirmed by DNA sequencing, which was exactly the same as designed; RT-PCR results showed that the FS gene transcription within 3AO human ovarian cancer cells were suppressed after transiently transfected the pLKO.1-FS-shRNA recombinant plasmids, and the interference rate on mRNA expression of FS was 59%. Conclusion: The human FS gene pLKO.1-FS-shRNA recombinant plasmids was constructed successfully, which lays the foundation for further study of the biological function of FS gene.
2013 Vol. 21 (4): 231-233 [Abstract]（
45
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Wang Haiying
To construct a new biomarker with which could evaluate the potential detection of HPV16 infection by explore the relationship between the grade of cervical lesions and HPV16 DNA methylation. Methods:Seventy-three HPV16 postive samples were collected from women undergoing routine cervical cancer screening. Complete methylation data were obtained for L1 gene ORF and URR CpGs in all samples by bisulfite-pyrosequencing. The methylation result was compared with that of pathological examination to analysis the relationship the grade of cervical lesions and HPV16 DNA methylation. Results:In sites of 31, 37, 43, 52 and 58, there was a moderate methylation level in no lesion samples, but with a low methylation level cervical intraepithelial neoplasia(CIN) samples and a high methylation level in Cervical cancer(CA) samples. The statistic analysis showed a significant difference among the normal and CIN and CA samples. There were significant differences among normal, CIN1, CIN2, CIN3 and CA samples in sites of 3887, 3927, 3941 and 5602. Besides, a significant difference existed between normal and CA sample or between CIN and CA samples in sites of 5608, 5709, 5611, 5617, 5762, 6367 and 6389. Conclusion: HPV16 DNA methylation level is associated with the grade of cervical lesions. And HPV16 DNA methylation level in clinical samples could be a biomarker of neoplastic cervical progression.
2013 Vol. 21 (4): 234-237 [Abstract]（
47
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Chen Ying,Yuan Yan,Tian Aiping
To explore the current status of intrauterine devices (IUDs) use in Sichuan Province. Methods: A multi-phase stratified cluster sampling method was used, and an epidemiological study with a cross-sectional, retrospective and prospective design was conducted in 2107 IUD users of three counties. Results: The top three IUDs used generally were Mutile-IUD, TCu380A IUD and YGCu IUD. There were differences in IUDs types among women in the three counties (P<0.001). The average continuation rate was 86.9%. About 75.3% of IUD insertions were operated by providers of the township level. The top three failure outcomes were extrusion, removal due to medical reasons and downward dislocation, accounting for 4.7%, 2.8%, 2.7%, respectively. One percent of cases suffered pregnancy with device. The training of the operators within 5 years was the protective factor of the extrusion(OR=0.817, P=0.007). Conclusion: Promotion of the clinic pathway system of IUD operation and construction of the standard operating procedure of different types of IUDs could improve its service qualities.
2013 Vol. 21 (4): 238-241 [Abstract]（
38
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Zhang Li,Liu Jing,Zhang Yixin,Li Guozheng,Wang Jingcun,Gao Zhangquan,Tian Zhaohui,Liu Xiaoqun,Yao Guanfeng
To explore the prevalence rate and causes of infertility in rural couples of childbearing age in Hebei Province in order to provide information for infertility etiology and prevention. Methods: According to the WHO definition of infertility, a stratified cross-sectional sampling method was used, and couples of childbearing age who were planning to become pregnant in 5 counties of Hebei Province were selected. Results: A total of 19 588 couples were recruited. There were 2256 infertile couple (11.5%). And the female factors accounted for 26.4%. Ovulation disorder was the most common factor for the female primary infertility, and tubal factor for the secondary infertility. The male factors accounted for 34.5%. The most common factor for either the primary infertility or the secondary one was semen abnormality. Conclusion: The prevalence rate of infertility is high in rural areas of Hebei Province. Therefore, the traditional childbearing concept of rural couples of childbearing age should be changed and the diagnosis and treatment for infertility should be standardized.
2013 Vol. 21 (4): 242-244 [Abstract]（
41
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Liu Bei
To explore the status of job burnout and its influencing factors among medical personnels in family planning service centers in order to provide basic data for intervention. Methods: A questionnaire survey was carried out among 372 medical personnels in family planning service centers in Jinan. Results: There is a certain degree of job burnout among medical personnels in family planning service centers. The demographic data showed that there were significant differences on the dimension of job burnout and scores in occupation, work experience, income, job title and organization type. Multiple regression showed that main influence factors of job burnout were income, organization type, job title and work experience. The total contribution rate was 34.25%. Conclusion: The effective preventive measures should be provided although the job burnout among medical personnels in family planning service centers is not serious.
2013 Vol. 21 (4): 245-248 [Abstract]（
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Yang Yongjun,Li Ming,Chen Dan,Chen Yumei,Liu Hui,Bao Jie,Cao Lixing ,Niu Chengling
To analyze the results of qualitative evalution among clinic labs of participating counties from 2010 to 2011 in family planning system in Henan Province in order to improve the quality of laboratory examinations and the ability of family planning services. Methods: Fifty samples of QC Blood including three kinds of parameters (complete blood count, routine chemistry examination and immunological examination) were collected and evaluated twice per year. Results: PT scores and passing rates of each parameter in 2011 were all significantly higher than those in the 2010. Conclusion: After the guidance of the provincial branch center and the qualitative evalution, abilities of laboratory examinations were all improved obviously.
2013 Vol. 21 (4): 249-250 [Abstract]（
39
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Zhong Chunli,Song Zhaohui,Xin Limei,Li Sha,Tang Chunye
To explore the effectiveness, security and feasibility of tubal sterilization with single-port laparoscopic bipolar coagulation. Methods: Some indicators during operation and follow-up were assessed in 618 women. Results: The tubal sterilization with single-port laparoscopic bipolar coagulation conducted in 604 women (97.73%) successfully. Unilateral tubal sterilization conducted in six women (0.97%) because of pelvic cavity adhesion. The operation failed in 8 women (1.29%) for failing in puncture or grave pelvic cavity adhesion. One women became pregnant 1 year after the operation. The blood loss was less than 5ml in 613 women (99.19%). A total of 589 women (96.56%) experinced lower abdominal pain 5 to 10 seconds after the electric coagulation began and relieved after 10 seconds. The adhesion release conducted in 6 women (0.97%). The mild to moderate complications were found in 17 women (2.75%). The average time of the operation was 19.36 minutes, and the average time of postoperative observation was 30.52 minutes. Some women who experienced abdominal pain, low back pain and menstruation changes 1, 3, 6, 12 months after the operation and recovered after the expectant treatment and psychological guidance. The satisfaction rate was 97.23%. Conclusion: Tubal sterilization with single-port laparoscopic bipolar coagulation is a safe, effective and acceptable method.
2013 Vol. 21 (4): 251-254 [Abstract]（
59
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Liu Jin,Zhu Wenjie,Zhou Yonghong,Fu Zhihong,Li Xuemei,Tang Xuelian
To explore the changes of endometrial receptivity in infertile women who were treated by oral contraceptive pills due to an elevated basal follicle stimulating hormone (FSH). Methods:Fifteen infertile women with elevated FSH were administrated with oral contraceptive pills for three cycles. The endometrial biopsies were performed in the mid-luteal phase before (group 1) and after (group 2) the therapy. The parameters of endometrial receptivity including the development of pinopodes and biochemical markers, e.g. leukemia inhibitory factor (LIF), vascular endothelial growth factor (VEGF), integrin β3, were compared. Results:All of 15 mid-luteal biopsies (100.0%) were in-phase in group 2. However, only 9 out of 15 (60.0%) of biopsies showed the in-phase endometria in group 1. The synchronization of endometrial development was significantly higher in group 2. The fully developed pinopodes rate was also higher in group 2 (57.8%) than in group 1 (35.6%, P＜0.05). The mean LIF and integrin β3 levels were 2.41±0.50 and 2.39±0.41 in group 2, significantly higher than 1.91±0.46 and 2.10±0.26 in group 1 (P＜0.05). Conclusion: Oral contraceptive pills administration improved endometrial receptivity and synchronous development in women with elevated basal FSH.
2013 Vol. 21 (4): 255-259 [Abstract]（
70
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Peng Wenpai,Ma Yuju,Huang Yanhua,Han Dingying,Xiao Min,Lin Xujun
To investigate some sensitive indicators for prediction of incomplete medical abortion fin early stage, and discuss intervention measures of high-risk women. Methods: The transvaginal color Doppler sonography was used to test the blood signal of the gestational sac implantation site and the resistance index (RI) value of its spiral artery in 196 health women who experienced medical abortion. Then 60 high-risk women determined with RI value were divided into two groups (30 women in each group). Women were administered with mifepristone (mifepristone treatment group) and Shenghua Decoction (Shenghua Decoction treatment group). The outcome of the medical abortion was assessed. Results: For patients of complete abortion, the blood signal of the gestational sac implantation site disappeared or the RI value significantly increased after the gestational sac discharge (P<0.01). For the patients of incomplete abortion, the change of the RI value was not statistically significant (P>0.05). The spiral arterial RI value of the implantation site after the gestational sac discharge was less than 0.6, which suggested that it was highly possible of incomplete medical abortion, and the predicting accuracy rate was 96.43%. After treatment, the complete abortion rate of the mifepristone treatment group (63.3%) was significantly higher than that of the Shenghua Decoction treatment group (13.3%, P<0.01). Conclusion: The transvaginal color Doppler sonography can be used for prediction of the incomplete medical abortion in the early stage. The high-risk incomplete abortion is suggested when RI is less than 0.6. And for the high-risk women, supplement with mifepristone after routine method of medical abortion can effectively prevent incomplete abortion.
2013 Vol. 21 (4): 260-261 [Abstract]（
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Qian Jufen,Tao Aiqun
To compare the clinical effect of loop electrosurgical excision procedure (LEEP) and cold-knife conization(CKC) for cervical intraepithelial neoplasia (CIN). Methods: A total of 164 patients suffering from CIN were randomly divided into LEEP group (n=82) and CKC group (n=82). The clinical effect, changes of pathological grades, postoperative complications and recurrences were assessed. Results: Compared with CKC group, the operation time and the wound healing time were shorter, and intraoperatve blood loss was less in LEEP group (P all <0.05).There were no significant differences in the consistent rate of pathology diagnosis, reduction rate of pathological grades, incidence of infiltrating carcinoma between the two groups (P>0.05).The rate of postoperative cervical tube adhesion in LEEP group was significantly less than that in CKC group (P<0.05).The recurrence rate in LEEP group and CKC group was 9.8% and 7.3%, showing no significant difference (P>0.05). Conclusions: LEEP is supperior to CKC in treating CIN for its safety and effectiveness as well as fewer complications.
2013 Vol. 21 (4): 263-265 [Abstract]（
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