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Li Donghua, Wu Jie
Objective: Osteoporosis is a condition characterized by low BMD and micro-architectural change in bone tissue. The risk of osteoporosis is partly determined by genetic factors. Some studies investigated the role of C677T polymorphism of methylenetetrahydrofolate reductase (MTHFR) in postmenopausal osteoporosis. However, the relationship between MTHFR polymorphism and BMD is still controversial. So a meta-analysis was performed to evaluate the association of MTHFR and BMD in postmenopausal women. Methods: MEDLINE, Web of Science, Scopus and CNKI were retrieved for all publications relating to MTHFR polymorphism and BMD in postmenopausal women. We performed this meta-analysis involving 5833 subjects. Results: Five eligible studies were selected for meta-analysis. These articles all studied the association of MTHFR polymorphism and the femoral neck and lumbar spine BMD in postmenopausal women. It suggested that the postmenopausal women with the TT genotype had lower femoral neck BMD than that of the CC/CT genotype women, and the weighted mean difference (WMD) was -0.01 g/cm2 [95% confidence interval (CI): -0.01, -0.01, P＜0.0001]. Individuals with TT genotype tended to have somewhat lower BMD of lumbar spine, but the difference was not statistically significant. In random effects model, the WMD between the TT and TC/CC genotypes was -0.01 g/cm2 (95% CI -0.04, 0.01, P=0.32). Conclusions: The C677T polymorphism of the MTHFR gene is associated with BMD of femoral neck in postmenopausal women. The women with the TT genotype of MTHFR gene have lower BMD, suggesting that TT genotype may serve as a risk factor for postmenopausal osteoporosis.
2012 Vol. 20 (11): 731- [Abstract]（
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Wang Cong, He Zhixu, Zhou Congrong
Objective: To investigate whether the marrow mesenchymal stem cells (MMSCs) could differentiate into spermatogenic cells in a rat model of male infertility. Methods: The frozen MMSCs-GFP were recovered and cultured, and then transplanted into the seminiferous tubule of the right testis of rat model of spermatogenic cells injure established with Busulfan (50mg/kg), with the left testis untreated as self control. The testes were collected 8 weeks later. The testicle weight index, testicular histology and donor cell distribution were observed. Immunofluorescence was employed to test the germ cell-specific marker c-kit expression. Results: The testicle weight index of MMSCs-GFP transplanted testis was comparable to that of normal rat testes (P>0.05), but significantly different from that of self-control testis (P<0.01). No local or systemic inflammartory reaction was observed, while the spermatogenic epithelium was regenerated in the the MMSCs-GFP transplanted testis. Fluorescence microscopy showed that most of MMSCs-GFP moved to the basilar zone of seminiferous tubule. The c-kit expression in the seminiferous tubule of the MMSCs-transplanted testis was significantly different from that of the self-control testis. Conclusion: Cryopreservation did not affect the proliferation ability of MMSCs. MMSCs transplanted into convoluted seminiferous tubule could differentiated into spermatogenic cells.
2012 Vol. 20 (11): 736- [Abstract]（
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Yang Shangwu, Huang Hao, Wang Xiaojie, Zhu Xiulan, Xiu Liangchang, Xing Fuqi
Objective: To explore the cortical granule (CG) change and parthenogenetic activation during mouse oocyte aging. Methods: Female mice, 8-10 weeks of age, were induced to superovulation with pregnant mare serum gonadotropin (PMSG; 10U, i.p.) followed 48 h later by hCG (10 U, i.p.). Oocytes were retrieved at different time intervals (MII+0h, +6h, +12h, +18h) (in vivo aging) or MII+0h oocytes cultured for different time periods (+6h, +12h, +18h) (in vitro aging). CG distribution and release were assessed by fluorescene isothiocyanate-labelled Lens culinaris agglutinin (FITC-LCA) staining and laser confocal microscopy. Spontaneous parthenogenetic activation and fragmentation of oocytes were observed after anther 8h of culture of different in vivo aging groups. Results: Spontaneous cortical granule exocytosis (CEG) and CG inward movement rates in MII+12h, MII+18h groups were statistically higher than those in MII+0h, MII+6h groups in both in vivo and in vitro aging oocytes. Spontaneous CGE and CG inward movement in M+18h group was lower in in-vitro aging group than those of in vivo aging counterpart. Spontaneous parthenogenetic activation and oocyte fragmentation ratios after further 8h of culture were higher in MII+12h,MII+18h groups than those in MII+0h, MII+6h groups. Conclusion: Spontaneous CGE and CG inward movement increased during oocyte aging both in vivo and in vitro. There were also increased parthenogenetic activation and oocyte fragmentation ratios as oocyte aged. All these may cause the low fertility of aged oocyte. Spontaneous CGE and CG inward movement may be accelerated by maternal environment changes.
2012 Vol. 20 (11): 742- [Abstract]（
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Zhou You, Zhang Ying, He Dian, Ji Ning, Pang Cheng, Ren Shanshan, Xi Maomao, Mao Qunxia, Cheng Yiminsup>2,*
Objective: To study the current situations of self-reported symptoms of reproductive tract infections (RTIs), health-seeking behaviour and the related influencing factors in rural married women in Shaanxi Province. Method: A questionnaire survey was conducted on 20,878 rural married women at 20-49 years of age selected by stratified clustering sampling in Shaanxi Province. Results: 14,482(69.5%) women reported at least one RTIs symptom in the past 6 months and 8,533(59.0%) of them once visited a doctor. The results of multiple Logistic regression showed that age (OR=0.924), occupation (OR=1.223), education level (OR=1.143), family yearly income (OR1=1.250, OR2=1.101), RTIs knowledge (OR=1.725) and number of RTIs symptoms (OR=1.107) were related to health-seeking behavior. Conclusion: The prevalence of self-reported RTIs symptom was high among this population, but their health-seeking behavior was relatively poor and affected by multiple factors. Health education and gynecological census should be strengthened in the future.
2012 Vol. 20 (11): 746- [Abstract]（
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Yu Yanjin, Zhuang Yaling, Qian Zhida, Chen Xiuying, Huang Lili
Objective: To study the acceptability of the medical abortion and its related factors. Methods: A questionnaire survey was conducted on 1,342 women who sought an abortion selected by stratified cluster random sampling from hospitals and family planning service stations. 1305 of those who provided valid responses were divided into 2 groups according their choices: 331 women in medical abortion (MA) group, and 974 women in surgical abortion (SA) group. Visual analog scale was used to evaluate the acceptability of the medical abortion. Results: 98.5% of respondents in the MA group were at 20-39 years of age, a significantly higher percentage than that of the SA group. The education level and social-economic conditions were significantly higher in MA group than in SA group. Age and education level were the influencing factors of abortion choice. The information on abortion from the medical personnel and the health providers had a negative effect on the choice of MA. For 108 participants with a history of both medical and surgical abortions, there was no significant difference in the satisfaction with these 2 methods. Conclusions: Medical abortion was more acceptable in the patients who are better educated. There was no significant difference in the acceptability of the two abortion methods in the abortion clients. The medical personnel and the health providers are usually dominant in choosing the abortion method.
2012 Vol. 20 (11): 749- [Abstract]（
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Wu Junchao, Hu Aorong,Yang Hongqing, Liu Junmin, Shi Jianhua, Wang Maofang, Deng Yun , Yang Guohua
Objective: To review the situation in the medical identification of disabled children in the past 10 years, hopefully providing evidence for the improvement of medical identification and prevention of disabled cases. Methods: The data of 2,798 children that applied for identification of disability in Suizhou from 2002 to 2011 were retrospectively studied. Results: The number of application for the medical identification of a disability was increasing by year. Problems in nervous system, cardiovascular system and motor system ranked at the top three in non-hereditary diseases, while polygene heredity diseases were the the top cause of genetic disability. More causes of disability were documented year by year. The top 5 disabilities were mental retardation, cerebral palsy, congenital heart disease, epilepsy, and cerebral hyperplasia. Conclusion: More and more cases of disability were submitted for the medical identification and documented in past 10 years, and the causes of disability are of more diversity. The procedures and protocols for medical identification should be improved accordingly. New technologies are suggested to be developed and applied, for the identification and prevention of disability.
2012 Vol. 20 (11): 753- [Abstract]（
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Chu Guanghua, Zou Yan, Wu Shangchun, Su Cunmei, Tian Aiping, Huang Zirong, Fang Aihua, Wang Xiaoye, Wang Cunping, Liu Jing, Ding Yuehong, Zhang Jue, Zhang Hongyan, Li Cunping
Objective: To study the psychological and mental health status of women of reproductive age at the removal of intrauterine device (IUD), and explore the effect of the standardized surgical operation on women's psychological state. Methods: A comparative, multicenter clinical observational study was performed. The representative sample was selected from six provinces' medical institutions. In each province one provincial health service center and four county-level family planning service stations were selected by the random cluster method, and randomly assigned to intervention and control groups. In intervention group the IUD was removed with the standard operating procedures, while in control group the surgical operation was the daily-used technique in each individual institution. A total of 970 eligible women were recruited from 2008 to 2009. Women underwent psychological evaluation with symptom checklist 90 (SCL-90) before intrauterine device removal and at post-operative follow-up one month later, respectively. Results: The pre-operative scores of SCL-90 of the studied women were all higher than the post-operative ones (P<0.05). The post-operative scores of SCL-90 of the women in the intervention group were lower than those of the control group (P<0.05). Conclusion: The procedure of IUD removal is safe, simple, effective, reversible and acceptable, but it, as a source of stress, could have some effect on the psychological state of women. Standardized surgical operation could have a positive effect on the mental health status of women.
2012 Vol. 20 (11): 757- [Abstract]（
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Zhuge Ting, Huang Zirong, Li Bin
Objective: To study the effect of conservative treatments for abortive remnants of induced abortion, and probe into the influencing factors related to the successful removal of the remnants. Methods: A total of 101 patients with abortive remnants after induced abortion were selected by simple random sampling, and randomly divided into 4 groups, with 3 groups treated with different dosages of mifepristone (50 mg/d×28d, 100 mg/d×14d, and 200mg/d×7d)and 1 control group. Based on the treatment outcomes, the patients were subgrouped into cured group and uncured group. The parameters of age, weight, gravidity, parity, bleeding time after treatment, baseline β-hCG (human chorionic gonadotrophin) levels, and the size of abortive remnants measured by B-mode ultrasonic diagnostic equipment were analyzed. Rank sum test and logistic regression method were used to analyze the correlated factors with successful treatment of abortive remnants of induced abortion with mifepristone. Results: The group with mifepristone 200mg/d×7d showed the most effective results (P<0.05). Compared with the uncured group, cured group had lower levels of serum β-hCG, smaller abortive remnants, shorter bleeding time after treatment, greater resistance index of blood flow (P<0.05 for all above factors). Logistic regression analysis showed that dosage of mifepristone, baseline β-hCG, the average size of the abortive remnants, and resistance index of blood flow status of the remnant tissue were closely correlated to the treatment outcomes (P<0.05). Conclusion: Mifepristone was effective in the treatment of incomplete induced abortion, and it was most effective when used at a short-term high dose. The baseline β-hCG, size of the abortive remnants and resistance index of bleed flow of the abortive remnants could predict the treatment outcomes.
2012 Vol. 20 (11): 762- [Abstract]（
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Zhou Shiyuan, Bo Liwei, Wang Yanli, Niu chengling,Xie Jiansheng, Chai Jian
Objective: To investigate the cervical condition and genotypes of human papillomavirus (HPV) infection and explore the relationship between different genotypes of HPV infections and cervical epithelial lesions. Methods: Cervical exfoliated cells were collected from 862 outpatients and examined by thin-cytologic test, and the cervical cytological conditions were evaluated by The Bethesda System (TBS) as negative for intraepithelial lesion or malignancy ((NILM) or different degrees of lesions. Low-density gene chip-based detection system for HPV genotypes (15 types of high-risk HPVs, 5 types of low-risk HPVs and CP8304) was employed to detect the HPV infections. Result: 19 of 21 HPV genotypes were detected in 862 specimens. The most common HPV genotypes were HPV11, 6, 42 and 16 in NILM group, and HPV16,52 and 58 in cervical lesion group. The detection rate of HPVs in NILM and lesion group were 11.28% and 64.66%, respectively. The constituent ratios of single and multiple infections of HPV genotypes were 75.86% and 24.14% in NILM group, and 71.11% and 28.89% in lesion group, respectively. The high-risk HPV genotypes were the most prevalent HPV infections (88.71%) in lesion group. The distribution of different HPV genotypes varied with the different degrees of cervical lesions. Conclusion: There was a close relationship between the multiple infection of HPV genotypes and the development of cervical epithelial lesions. Infection of HPV(s) increased with the grade of cervical lesions.
2012 Vol. 20 (11): 765- [Abstract]（
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Wang Wei, Wang Aiming, Jiang Wen, et al
2012 Vol. 20 (11): 787- [Abstract]（
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