To explore the clinical application value of the special staining technique of the cervical exfoliated cells for screening the cervical lesions. Methods: A total of 2000 women who underwent cervical lesion screening in the hospital from August 2022 to May 2023 were selected in this study. All these women had undergone the detections of the cervical exfoliated cell special staining technology (FRD), human papillomavirus (HPV) and thinprep cytology test (TCT) for their cervical lesion screening, the colposcopy was performed on the women with the positive screening result of one of the three methods, and the cervical biopsy was performed in the women with the cervical lesions by colposcopic examination. The detection efficacy and the area under the curve (AUC) of the receiver operating characteristic curve were compared among the women with FRD detection, the women with TCT detection alone, the women with HPV detection alone and the women with TCT combined with HPV detections based on the histopathological findings as the gold standard of the cervical lesion. Results: The sensitivity, the specificity, the Kappa value and the AUC of the FRD detection for the cervical lesions were 83.8%, 90.0%, 0.618 and 0.869, respectively. The sensitivity, the specificity, the Kappa value and the AUC of the TCT detection alone for the cervical lesions were 65.1%, 89.5%, 0.485 and 0.773, respectively. The sensitivity, the specificity, the Kappa value of the HPV detection alone for the cervical lesions were 94.6%, 71.7%, 0.387 and 0.832, respectively. The sensitivity, the specificity, the Kappa value and the AUC of the TCT combined with HPV detections for the cervical lesions were 98.9%, 70.2%, 0.391 and 0.846, respectively. Conclusion: The special staining technique of the cervical exfoliated cells for screening the cervical lesions has better effective, and which can be used to cervical lesion screening in the grassroots level of the medical institutions.