To understand the status of pain disclosure of women with artificial insemination, and to analyze its influencing factors. Methods: A total of 148 women with artificial insemination infertility admitted to the hospital from April 2021 to April 2023 were selected in this study. The general data questionnaire, the distress disclosure index (DDI), the perceived social support scale (PSSS) and the Locke-Wallace marriage adjustment test scale (MAT) were used for investigation. Pearson correlation coefficient model was used to analyze the correlation between the scores of IPSSS and MAT of the women with artificial insemination and the level of their pain disclosure. And multiple linear regression model was used to analyze the influencing factors of the pain disclosure of the women with artificial insemination. Results: A total of 148 questionnaires were distributed and 138 valid questionnaires were returned, with an effective rate of 93.2%. The DDI score of 138 women was 35.25±5.48 points, and the low level of DDI score accounted for 14.5%, the medium level of DDI score accounted for 73.2% and the high level of DDI score accounted for 12.3%. The score of the PSSS and the MAT of the women with artificial insemination were 53.15±9.67 points and 99.56±12.21 points. Univariate analysis showed that there was statistically significant difference in the pain disclosure score among the women with different age, among the women with different family per capita monthly income, among the women with different place of residence, between the women with and without artificial insemination by their husband,  and among the women with different artificial insemination methods (P<0.05). Pearson correlation analysis showed that the PSSS and MAT scores of the women with artificial insemination were positively correlated with their DDI score (all P<0.001). Multiple linear regression analysis showed that the family per capita monthly income, whether the artificial insemination by husband or not, the PSSS score and the MAT score of the women with artificial insemination had the positive impacts on the level of their painful disclosure, and the number of artificial insemination of the women had the negative effect on the level of their pain disclosure (all P<0.05). Conclusion: The pain disclosure of the women with artificial insemination is at a medium level in this investigation. The family per capita monthly income, whether the artificial insemination by husband or not, the number of artificial insemination, the social support and the marital intimacy of the women with artificial insemination are the important influencing factors of their pain disclosure, so which should be paid attention to in clinic and the targeted nursing interventions should be given, so as to relieve the pain disclosure level and promote the physical and mental health of the women.