To investigate the influence of general anesthesia combined with epidural anesthesia used in laparoscopic myomectomy of patients on their plasminogen activator inhibitor-1(PAI-1), tissue Plasminogen activator(t-PA), D-dimer(D-D) and prognosis. Methods: 96 patients with uterine fibroids who wanted laparoscopic myomectomy were randomly selected and were divided into two groups(48 cases in each group) from March 2018 to March 2023. The patients in the control group were given simple general anesthesia, and the patients in the study group were given general anesthesia combined with epidural anesthesia. The anesthetic effect, and the fibrinolysis function, the hemodynamics and the sex hormones levels before and after intervention, and the adverse reactions rate and the prognostic indicators of the patients were compared between the two groups. Results: The levels of PAI-1(26.52±3.54μg/L), t-PA(15.67±2.89μg/L), and D-D(155.62±21.47μg/L) of the patients in the study group after anesthesia were significantly lower than those(41.32±4.82μg/L, 22.39±3.16μg/L, and 189.41±22.59μg/L) of the patients in the control group. The values of heart rate and mean arterial pressure, and the levels of follicle-stimulating hormone and luteinizing hormone of the patients in the study group were significantly lower than those of the patients in the control group. The incidence of adverse reactions(10.4%) and the recurrence rate(4.2%) of the patients in the study group were significantly lower than those(29.2% and 16.7%) of the patients in the control group. The anesthesia effect of the patients in the study group was significantly better than that of the patients in the control group. The menstruation recovery rate(95.8%) of the patients in the study group was significantly higher than that(81.3%) of the patients in the control group(all P<0.05). Conclusion: The general anesthesia combined with epidural anesthesia used in laparoscopic myomectomy of the patients can improve their fibrinolytic function, hemodynamic indexes and sex hormone indexes, and which has better anesthetic effect, lower adverse reactions and better prognosis.