To investigate the effects of perioperative heat preservation measures during laparoscopic ectopic pregnancy surgery of patients on their core temperature and stress response. Methods: A total of 150 patients who wanted elective laparoscopic ectopic pregnancy surgery were selected and were divided into two groups (75 cases in each group) from January 2020 to August 2022. The patients in the observation group were given perioperative composite heat preservation measures, and the patients in the control group were given perioperative routine heat preservation measures. The values of intraoperative core temperature, heart rate, blood pressure, coagulation indexes, and the levels of serum epinephrine and norepinephrine concentrations of the patients in the two groups were analyzed. Results:  The core temperature of the patients in the observation group at 1h after anesthesia (36.13±0.82℃) and at the end of operation (36.17±0.78℃) were significantly higher than those (35.01±0.82℃ and 34.87±0.84℃) of the patients in the control group. The values of intraoperative HR (71.4±7.5 beats/min), systolic blood pressure (126.6±10.1 mmHg), and diastolic blood pressure (72.9±7.9 mmHg), the levels of AD (104.62±20.55 pmol/L) and NE (160.77±37.03) pmol/L), the extubation time (27.29±6.90 min), and the anesthesia recovery time (36.52±7.13 min) of the patients in observation group were significantly lower than those (79.5±8.9 times/min, 141.8±19.1 mm Hg, 84.0±9.4 mm Hg, 119.43±31.91) pmol/L, 179.38±41.22 pmol/L, 25.94±6.33 min, and 33.79±6.84 min) of the patients in the control group. The values of the coagulation function indexes of the patients in the observation group were significantly lower than those of the patients in the control group (all P＜0.05). Conclusion: Active perioperative heat preservation measures for the patients during laparoscopic ectopic pregnancy surgery can prevent their hypothermia and reduce their stress response, and which is beneficial to their postoperative recovery.