|
|
|
| Expressions and clinical significances of Th1/Th2 cytokine in peripheral blood, and Tim-3 and PD-1 in peripheral blood mononuclear cells of women with unexplained recurrent miscarriage |
| Hainan Western Central Hospital, Danzhou, Hainan Province,571700 |
|
|
|
Abstract To investigate the expressions and clinical significances of T helper cell 1/T helper cell 2 (Th1/Th2) in peripheral blood, and T cell immunoglobulin mucin-3 (Tim-3) and programmed death factor 1 (PD-1) in peripheral blood mononuclear cells (PBMC) of women with unexplained recurrent miscarriage (URSA). Methods: A total of 61 women with URSA were collected in study group, and 61 women who were preparing for pregnancy after the physical examination were collected in control group from January 2019 to December 2021. The condition of Th1/Th2 cells, and the levels of interleukin-4 (IL-4) and interferon-γ (INF-γ) in peripheral blood of the women in the two groups were detected. The density gradient centrifugation method was used to separate PBMC, and the expressions of Tim-3 and PD-1 in PBMC of the women in the two groups were detected. The correlations of the above various indicators among each other were analyzed, and their values for diagnosing URSA were also analyzed. Results: The values of Th1 cell subsets, the Th1/Th2 ratio, the IFN-γ level, and the IFN-γ/IL-4 ratio of the women in the study group were significantly higher than those of the women in the control group, but the Th2 cell subsets values and the IL-4 level of the women in the study group were significantly lower. The expression values of Tim-3 (3.12±0.53) and PD-1 (2.69±0.38) in PBMC of the women in the study group were significantly lower than those (4.79±0.82 and 4.05±0.42) of the women in the control group. The Th1/Th2 cell ratio and IFN-γ/IL-4 ratio in peripheral blood of the women with URSA were negatively correlated with their expression values of Tim-3 and PD-1 in PBMC, while the Tim-3
expression value of the women with URSA was positively correlated with their PD-1 expression value (all P<0.05). The areas under the receiver operator characteristic curve of the Th1/Th2 ratio, the IFN-γ/IL-4 ratio, and the expression values of Tim-3 and PD-1 of the women for diagnosing their URSA were 0.709, 0.642, 0.691, and 0.614, respectively. Conclusion: The balance of Th1/Th2 of the women with URSA presents the advantage of Th1, and which is accompanied by low expressions of Tim-3 and PD-1 in PBMC. The imbalance of Th1/Th2 of the women with URSA is related to the low expressions of their Tim-3 and PD-1, and which may be involved in the occurrence and development of URSA and can be used as the potential targets of clinical diagnosis and treatment for URSA.
|
|
|
|
|
|
|
|
|
