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| Influence of the down-regulated expression of protein kinase membrane associated tyrosine/threonine 1 on the proliferation, migration, and invasion of cervical cancer cell |
| Huai 'an Hospital, Huai 'an, Jiangsu Province, 223200 |
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Abstract To investigate the influence of thedown-regulated expression of protein kinase membrane associated tyrosine/threonine 1 (PKMYT1) on the proliferation, migration, and invasion of cervical cancer cell. Methods: UALCAN platform was used to analysis the expression of PKMYT1 in the cervical cancer tissues. The quantitative real-time PCR (RT-qPCR) was applied to examine the PKMYT1 expression in END1/E6E7 cell and in cervical cancer cell. The c-33A cell and SiHa cell were divided into control group (transfection of siRNA-NC) and siRNA-PKMYT1 group (downregulation of PKMYT1 expression). RT-qPCR and western blot were applied to examine the mRNA and protein expressions of wingless-type MMTV integration site family member 1 (WNT1), β-catenin, and c-Myc. MTT, scratch test, and Transwell chamber assays were performed to detect the proliferation, migration, and invasion of the cells. Results: PKMYT1 expression in the cervical cancer tissues and in the cells was up-regulated (P<0.05). The expression levels of Wnt1, β-catenin, and c-Myc in the c-33A and SiHa cells in siRNAPKMYT1 group were significantly lower than those in the control group, and the abilities of proliferation, migration, and invasion of the c-33A and SiHa cells in siRNA-PKMYT1 group were significantly lower than those of the c-33A and SiHa cells in the control group (all P<0.05). Conclusion: The silence of PKMYT1 can inhibit the proliferation, migration, and invasion progressions of the cervical cancer cell, and can decrease the activity of Wnt/β-catenin signaling pathway, which indicates that PKMYT1 maybe play a tumor suppressor of the cervical cancer.
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