To investigate the expressions and clinical significances of carbohydrate antigen 125 (CA125), aldehyde dehydrogenase 1 (ALDHl), sex determining region Y related high mobility group box 2 (SOX2), and alpha fetoprotein (AFP) in ovarian epithelial tumors tissues. Methods: 192 patients with ovarian epithelial tumors confirmed by biopsy and histopathology from November 2015 to January 2018 were collected as the study subjects. The expressions of CA125, ALDH1, SOX2, and AFP in the ovarian tumor tissue (in group A) and in the ovarian tissues of adjacent tumor (in group B) of the patients were detected by immunohistochemistry. Results: The positive expression rates of CA125 (57.3%), ALDH1 (42.7%), SOX2 (62.0%), and AFP (48.4%) in group A were significantly higher than those (27.1%, 2.6%, 6.3% and 5.6%) in group B. The expressions of CA125, ALDH1, SOX2, and AFP of the patients with high tumor stage or high pathological grade had increased significantly (all P<0.05). All 192 patients were followed up for 5-7 years, with a median time of follow-up of 6.3 years. At the end of the follow up, 90 (46.9%) patients had died and 102 (53.1%) patients still survived. The 5-year survival rate of the patients with the positive expressions of CA125, ALDH1, SOX2, and AFP in the ovarian tumor tissue was significantly lower than that of the patients with the negative expressions (P<0.05). Conclusion: The expressions of CA125, ALDH1, SOX2, and AFP in ovarian epithelial tumor of the patients are abnormal and are all closely related to the tumor stage and the pathological grade of the ovarian epithelial tumor, and which can be used as the evaluating indexes for the prognosis of the patients.