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| Application effect of hemoglobin A2/F/A1c detection by high performance liquid chromatography for screening β thalassemia |
| 1. Renhe District People's Hospital of Panzhihua, Panzhihua, Sichuan Province, 617061；2. Panzhihua Central Hospital |
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Abstract To study the application of hemoglobin A2/F/A1c detection for screening β thalassemia. Methods: The clinical data of 2642 clients who had accepted premarital examination from July 2020 to July 2022 were collected. The automatic analyzer was used to detect the index of red blood cells and abnormal hemoglobin of these clients. RDB method was used to analyze the genotypes of the clients withβ thalassemia. The HbA2 test was conducted by high performance liquid chromatography (HPLC), hemoglobin A1c A2/F/detection reagent (study kit), and VARIANT Ⅱ β thalassemia Short Program (control kit), and the effect of hemoglobin A2/F/A1c detection reagent for screeningβthalassemia of the clients was evaluated. Results: The β thalassemia of 2642 clients were screened, with the β thalassemia positive rate of 7.0%, including 177 clients with mild β thalassemia, 4 clients with intermediate β thalassemia, and 3 clients with severe β thalassemia. According to the retention time of abnormal Hb in HPLC detection, the proportion of total Hb, and the chromatographic graphic characteristics, 5 kinds of abnormal Hb were found. 7 kinds of genotypes of β thalassemia were found by RDB method, and CD41 42 (TCTT), 654 (CT), IVS Ⅱ CD17 (AT), and 28 (AG) of the clients with β thalassemia were the most common. The genotypes of the clients with severe βthalassemia was IVS Ⅱ 654 (CT) and the genotypes of the clients with intermediateβthalassemia was 28 (AG) homozygote. The positive, negative, and overall coincidence rates of study kit and control kit were 97.8%, 99.9%, and 99.7%, respectively. Conclusion：HPLC technology has less affected by human intervention and is suitable for large scale population screening. Hemoglobin A2/F/A1c reagent has the advantages of convenience, low cost, and high accuracy in detecting HbA2 level, and which has good application effect for screening β thalassemia in clinic.
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