To explore the clinical value of combined detections of serum β-human chorionic gonadotropin (β-hCG), interleukin-6 (IL-6), and alkaline phosphatase (ALP) of pregnant women for predicting their threatened abortion. Methods: A total of 82 pregnant women who received regular antenatal examinations and were diagnosed with threatened abortion were selected in research group from January 2019 to January 2020. All the pregnant women were treated with conventional symptomatic treatment. Another 82 pregnant women with normal antenatal examination were selected in control group during the same period. The serum β-hCG, IL-6, and ALP levels of the women in the two groups were detected. The pregnancy outcomes of the women in the research group were recorded. Receiver operating characteristic (ROC) curve was used to evaluate the diagnostic value of the combined levels of serum β-hCG, IL-6, and ALP of the women for the occurrence and the outcomes of their threatened abortion. Results: The levels of serum β-hCG and ALP of the women in the research group were significantly lower than those of the women in the control group, while the level of IL-6 of the women in the research group was significantly higher. Among 82 pregnant women with threatened abortion, 59 cases continued pregnancy and there were 23 cases with abortion after treatment. The levels of serum β-hCG and ALP of the women with continued pregnancy were significantly higher than those of the women with abortion, and the level of IL-6 of the women with continued pregnancy was significantly lower (all P＜0.05). The area under curve (AUC) of the serum β-hCG level, IL-6 level, ALP level, and the combined levels of serum β-hCG and ALP of the women for predicting the occurrence of their threatened abortion were 0.768, 0.797, 0.771, and 0.909, respectively, and the AUC of which for predicting the outcomes of their threatened abortion were 0.724, 0.771, 0.739, and 0.907, respectively (P＜0.05). Conclusion: The changes of the serum β-hCG level, IL-6 level, and ALP level of the pregnant women can predict the occurrence and outcomes of their threatened abortion, and the combined detections of β-hCG, IL-6, and ALP levels of the pregnant women can improve the diagnostic efficacy for their threatened abortion.