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| Analysis of the transcriptomics and the thermogenic activity changes of brown adipose tissue of pregnant mice with gestational diabetes mellitus |
| 1. Women’s Hospital affiliated to Nanjing Medical University (Nanjing Maternity and Child Health Care Hospital), Nanjing, Jiangsu Province, 210004；2. Jiangsu Second Normal University, Nanjing |
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Abstract To investigate the transcriptomics change of brown adipose tissue (BAT)of pregnant mice with gestational diabetes mellitus (GDM), and to study its influence on the thermogenic activity changes. Methods: The transcriptome expression profiles of 6 pregnant mice were analyzed by high-throughput sequencing, which included 3 normal pregnant mice in group A and 3 pregnant mice with GDM in group B. The differentially expressed genes of the mice were screening. The gene ontology (GO)and Kyoto Encyclopedia of Genes and Genomes (KEGG)analyses were performed. The expression of the uncoupling protein 1 (UCP1)of marker protein of the mice was detected by immunohistochemistry, Western blot, and real-time PCR in order to evaluate the thermogenic activity of BAT of the mice with GDM. Results: The high-throughput sequencing had identified 372 differentially expressed genes, of which, 255 genes of the mice with GDM were down-regulated and 117 genes were up-regulated. GO and KEGG analysis showed that the main targets of these differential genes of the mice were closely related to the abnormal lipid metabolism and the insulin signal transduction. Compared with those of the mice in group A, BAT of the mice in group B showed the increased lipid droplets, the loose texture, and the decreased UCP1 protein and gene expressions. Conclusion: The transcriptome profiles in BAT of the mice with GDM are significantly different from that of the mice with normal pregnancy. The thermogenic activity of the mice with GDM decreases, which may cause the glucolipid metabolism and insulin resistance disorders of these mice.
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