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| Inhibition of the migration, invasion, and tube formation of human chorionic trophoblast cells HTR-8/SVneo by miR-30c-5p regulating Notch1 expression |
| 1. School of Clinical Medicine, Weifang Medical University, Weifang, Shandong Province, 261053, 2. The 960th Hospital of the People’s Liberation Army Joint Logistics Support Force, Jinan, Shandong Province |
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Abstract To explore the mechanism of the inhibition of the migration, invasion, and tube formation of human chorionic trophoblast cells HTR-8/SVneo (HTR-8/SVneo)by miR-30c-5p regulating Notch1 expression, and to determine the potential role of miR-30c-5p in the pathogenesis of preeclampsia. Methods: The HTR-8/SVneo cells were cultured in vitro. The HTR-8/SVneo cell lines with miR-30c-5p overexpression or knockdown stabilized were constructed by lentivirus system. The HTR-8/SVneo cells lines were divided into group A (control group), group B (cells lines with miR-30c-5p overexpression), and group C (cells lines with miR30c-5p knockdown). The gene expressions of miR-30c-5p and Notch1 of the cells in the three groups were detected by quantitative real-time PCR (qRT-PCR). The expressions of Notch1 protein of the cells in the three groups were detected by Western blot. The migration, invasion, and tube formation ability of the cells in the three groups were detected by wound healing assay, Transwell method, and tube formation assay, respectively. Results: The expression level of miR30c-5p gene of the cells in group B was significantly higher than that of the cells in group A (P＜0.0001), the expression level of Notch1 protein of the cells in group B was significantly lower than that of the cells in group A (P＜0.01), and the abilities of migration, invasion, and tubular formation of the cells in group B were significantly lower than those of the cells in group A (P＜0.01, P＜0.001, and P＜0.05). The expression level of miR-30c-5p gene of the cells in group C was significantly lower than that of the cells in group A (P＜0.0001), the expression level of Notch1 protein of the cells in group C was significantly higher than that of the cells in group A (P＜0.001), and the abilities of migration, invasion, and tubular formation of the cells in group C were significantly higher than those of the cells in group A (P＜0.01, P＜0.05, and P＜0.01). Conclusion: MiR-30c-5p can decrease migration, invasion, and tube formation of HTR-8/SVneo cells by inhibiting the expression of Notch1, which is involved in the pathogenesis of preeclampsia.
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