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| Expressions of matrix metalloproteinase-2, myosin light chain kinase, and sulfotransferases 1A3 in uterine leiomyoma tissues of patients and their correlation with the uterine leiomyoma occurrence |
| 1. Meishan City People’s Hospital，Meishan, Sichuan Province, 620000；2. The Second People’s Hospital of Chongqing |
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Abstract To analyze the expressions of matrix metalloproteinase-2 (MMP-2), myosin light chain kinase (MLCK) and sulfotransferases 1A3 (SULT1A3) in uterine leiomyoma tissue of patients, and to study their correlation with the uterine leiomyoma occurrence. Methods: The uterine leiomyoma tissues and normal uterine tissues of 60 patients who had undergone surgical resection of uterine leiomyoma from January 2019 to June 2021 were collected. The mRNA expression levels of MMP-2, MLCK, and SULT1A3 were detected by RT-PCR, and the protein expressions of MMP-2, MLCK, and SULT1A3 were detected by immunohistochemistry. The correlation between the expressions of mRNA and protein of MMP-2, MLCK, and SULT1A3 and the occurrence of uterine leiomyoma was analyzed. Results: The mRNA relative expression levels of MMP-2 (1.61±0.23) and MLCK (11.87±1.60) in uterine leiomyoma tissues were significantly higher than those (1.05±0.09 and 6.14±1.02) in normal uterine tissues. The mRNA relative expression level of SULT1A3 (0.69±0.11) in uterine leiomyoma tissues was significantly lower than that (1.65±0.44) in normal uterine tissue. The positive rates of MMP-2 protein (68.3%) and MLCK protein (76.7%) in uterine leiomyoma tissues were significantly higher than those (25.0% and 26.7%) in normal uterine tissue, and the positive rates of SULT1A3 protein (61.7%) in uterine leiomyoma tissues was significantly were lower than that (100.0%) in normal uterine tissue (all P＜0.05). The mRNA expression of MMP-2 was positive correlation with the mRNA expression of MLCK. The mRNA expressions of MMP-2 and MLCK were negative correlation with the mRNA expression of SULT1A3. The protein expression of MMP-2 was positive correlation with the protein expression of MLCK. The protein expressions of MMP-2 and MLCK were negative correlation with the protein expression of SULT1A3 (all P＜0.05). Conclusion: The expression levels of MMP-2 and MLCK in the uterine leiomyoma tissue are high, and the expression level of SULT1A3 in the uterine leiomyoma tissue is low, and which have correlation each other. It is suggesting that the abnormal expressions of MMP-2, MLCK, and SULT1A3 may be closely related to the occurrence of uterine leiomyoma.
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