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| Correlation of the changes in vaginal flora and the expressions of fork head/winged helix transcription factor 3 and signal transducer and activator of transcription 5 in mononuclear cells of peripheral blood of women with premature rupture of membranes during the late pregnancy and their pregnancy outcomes |
| 1. Daxing District People's Hospital of Beijing, Beijing,102600; 2. Beijing Miyun District Hospital |
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Abstract To analyze the changes in vaginal flora and the expressions of fork head/winged helix transcription factor 3 (FoxP3) and signal transducer and activator of transcription 5 (STAT5) in mononuclear cells of peripheral blood, and the pregnancy outcomes of pregnant women with premature rupture of membranes (PROM) during the third trimester of pregnancy. Methods: The clinical data of 123 pregnant women with PROM (in group A) and 120 normal pregnant women during the third trimester of pregnancy (in group B) from June 2020 to December 2021 were collected. The distribution of microorganisms in vaginal secretions before childbirth, the expression levels of FoxP3 and STAT5 mRNA in mononuclear cells of peripheral blood, and the levels of serum IFN-γ, IL-12, IL-4, and IL10 of the women in the two groups were detected. The differences of the distribution of vaginal flora and the expression levels of FoxP3 and STAT5 mRNA of the women in the two groups were analyzed. Results: The proportion of lactobacillus (51.2%) of the women in group A was significantly lower than that (75.0%) of the women in group B. The proportions of gram-positive bacilli (15.5%), gram-positive cocci (13.0%), staphylococcus haemolyticus (21.1%), enterococcus faecalis (25.2%), and enterobacter cloacae (17.9%) of the women in group A were significantly higher than those (6.7%, 5.0%, 8.3%, 10.0%, and 7.5%) of the women in group B. The expression levels of FoxP3 (0.78±0.12) and STAT5 mRNA (1.04±0.22) in mononuclear cells of peripheral blood of the women in group A were significantly lower than those (1.26±0.34 and 1.75±0.42) of the women in group B (all P＜0.05). The levels of serum IFN-γ and IL-12 of the women in group A were significantly higher than those of the women in group B, and the levels of IL-4 and IL-10 level of the women in group A were significantly lower. In group A, the expression levels of FoxP3 and STAT5 mRNA in mononuclear cells of peripheral blood of the women with vaginal microecological imbalance were significantly lower than those of the women with normal vaginal microecological, and the expression levels of FoxP3 and STAT5 mRNA in mononuclear cells of peripheral blood of the women with poor pregnancy outcomes were significantly lower than those of the women with normal pregnancy outcomes (all P＜0.05). Conclusion: The expression levels of FoxP3 and STAT5 mRNA in mononuclear cells in peripheral blood of the pregnant women with PROM decrease, and are correlated with the changes of the vaginal flora and the pregnancy outcomes of these women, and which may be related to the mechanism of PROM.
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