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| Expressions of serum microRNA-543 and microRNA-135a of patients with intrauterine adhesion after hysteroscopic separation and their values for predicting the recurrence of intrauterine adhesion |
| Yiling District Maternal and Child Health Care Hospital, Yichang, Hubei Province, 443000 |
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Abstract To investigate the correlation between the serum microRNA-543（miR-543) and microRNA-135a（miR-135a) levels of the patients with intrauterine adhesion (IA) after hysteroscopic separation and their recurrence of IA, and to study the value of the miR-543 and miR-135a levels of the patients for predicting their recurrence of IA. Methods: 127 patients with IA who wanted hysteroscopic uterine adhesion separation were selected as the research objects from December 2019 to December 2020. According to the re-examination on the 3rd-7th day in the end of three menstrual cycles after the operation, the patients were divided into group A (34 cases with recurrence of IA) and group B (93 cases without recurrence of IA). Real-time fluorescent quantitative PCR （qRT-PCR) method was used to detect the serum levels of miR-543 and miR-135a of the patients at 24 h after operation in the two groups. Pearson method was used to analyze the correlation between the serum miR-543 and miR-135a levels of patients and the recurrence of their IA. Receiver operating characteristic（ROC) curve was used to analyze the predictive value of the serum miR-543 and miR-135a levels for the recurrence of IA. Logistic regression analysis was used to analyze the factors affecting the recurrence of IA after hysteroscopic surgical. Results: The course of disease and the serum Mir-135a level of the patients in group A were significantly more than those of the patients in group B, and the mir-543 level of the patients in group A was significantly lower (P<0.05). The serum Mir-543 level of the patients in group A was negatively correlation with their Mir-135a level (r=-0.539, P<0.05). The area under the curve (AUC), the specificity, and the sensitivity of the serum Mir-543 level for predicting the recurrence of IA were 0.897, 76.3%, and 91.2%, respectively. The AUC, the specificity, and the sensitivity of the serum Mir-135a level for predicting the recurrence of IA were 0.864, 92.5%, and 70.6%, respectively. The AUC, the specificity, and the sensitivity of serum Mir-543 level combined with Mir-135a for predicting the recurrence of IA were 0.947, 88.2%, and 94.1%, respectively. Mir-135a was an independent risk factor of the postoperative recurrence of IA (P<0.05), and Mir-543 was a protective factor of the postoperative recurrence of IA (P<0.05). Conclusion: Recurrence of IA after hysteroscopic treatment of the patients is related to the levels of their serum miR-543 and miR-135a, and the serum levels of miR-543 and miR-135a have certain predictive values for the recurrence of IA.
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