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| The expressions and significances of aquaporin 9 and hepatocyte growth factor in the placenta and fetal membranes of pregnant women with oligohydramnios |
| Haian Hospitai Affiliated to Nantong University, Jiangsu Province, 226600 |
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Abstract To investigate the expressions and significances of aquaporin 9 (AQP9) and hepatocyte growth factor (HGF) in the placenta and fetal membranes of pregnant women with oligohydramnios. Methods：The pregnant women were selected as the research subjects from August 2018 to October 2020. According to the characteristics of the amniotic fluid measured by B-ultrasound，these women were divided into the study group（65 cases with oligohydramnios） and control group（65 cases with normal amniotic fluid). RT-PCR and Western blotting were used to detect the mRNA and protein expression levels and differences of AQP9 and HGF in placenta and fetal membrane tissues. Results：The expression levels of AQP9 mRNA (1.64±1.74) and HGF mRNA (1.53±1.52) of the women in the study group were significantly lower than those (5.32±0.37 and 4.69± 0.42) of the women in the control group. The protein expression score of AQP9 (1.36±0.60 points) and HGF (2.14±0.46 points) of the women in the study group were significantly lower than those (5.32±0.29 points and 5.42±0.61 points) of the women in the control group. The positive rates of AQP9 (16.9%) and HGF (20.0%) in placental tissues of the women in the study group were significantly lower than those (83.1% and 86.2%) of the women in the control group (all P<0.05). The levels of AQP9 mRNA (1.52±1.23) and HGF mRNA (1.85±1.22) of the women in the study group were significantly lower than those (5.26±0.42 and 4.42±0.39) of the women in the control group. The expression scores of AQP9 (1.85±0.32 points) and HGF (2.03±0.21 points) of the women in the study group were significantly lower than those (5.03±0.319 points and 5.10±0.35 points) of the women in the control group, and the positive rates of AQP9 (18.5%) and HGF (26.2%) of the women in the study group were significantly lower than those (76.9% and 64.6%) of the women in the control group (all P<0.05). Conclusion：The mRNA and protein expressions of AQP9 and HGF in the placenta and fetal membranes of the pregnant women with oligohydramnios are low，which suggest that both of the mRNA and protein of AQP9 and HGF may have an important regulatory effect on the maternal-fetal fluid exchange and the intra-amniotic fluid absorption.
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