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Abstract To investigate the role of brain-derived neurotrophic factor (BDNF) and its receptor tyrosine protein kinase B (TrkB) in the release of the first polar body of oocytes. Methods: The expression of BDNF and its receptor TrkB in ovarian tissue during oocyte maturation was analyzed by gene microarray of Affymetrix, and the expressions of BDNF and its receptor TrkB in follicular tissue were analyzed by real-time quantitative PCR (RT-PCR). TrkB inhibitor K252a was used to study the role of BDNF in the first pole of oocyte release. Results: During the simulated maturation of oocytes, the expression of BDNF showed two peaks, the second peak was more significant than the first one. TrkB expression also showed two peaks, but both of them were very low. BDNF was expressed in cumulus cells, parietal granulosa cells, and oocytes, while TrkB was only expressed in oocytes. Different concentrations of BDNF (1, 3, 10, 30 ng/ml) increased the rate of the first polar body released from oocytes, and the concentration of BDNF (3ng/ml) increased the rate of the first polar body released from oocytes more significantly. In vivo experiments showed that different doses of K252a could inhibit the rate of the first polar body released of oocyte (P<0.05), but different concentrations of K252a inhibitor had no significant difference in the rate of the first polar body released of oocyte. Conclusion: BDNF plays an important role in the release of the first polar body of oocytes.
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