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| The change characteristic of intestinal flora in women with gestational diabetes mellitus, its correlation with inflammatory factors and T lymphocyte subsets, and its influence on pregnancy outcomes |
| 1.The First People’s Hospital of Hangzhou, Affiliated to Medicine School of Zhejiang University, Hangzhou, Zhejiang Province, 310006；2.Hangzhou Women’s Hospital of Hangzhou |
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Abstract To investigate the change characteristic of intestinal flora of pregnant women with gestational diabetes mellitus (GDM), to study its related inflammatory factors and T lymphocyte subsets of the women, and to analyze its influence on the pregnancy outcomes. Methods: From May 2019 to December 2020, 126 pregnant women were selected as the subjects, and were divided into group A (52 women with GDM) and group B (74 women with normal glucose tolerance) based on their results of glucose tolerance test during 24-28 gestational weeks. The characteristic of intestinal flora, the inflammatory factors levels, and the difference of T lymphocyte subsets of the women were compared between the two groups. And the correlation analysis was carried out. The influence of the characteristic of intestinal flora of the women on their pregnancy outcomes was also analyzed. Results: There was no significant difference in the number of fusobacteria colonies of the women between the two groups (P＞0.05). The numbers of bifidobacteria and lactobacillus of the women in group A were significant lower than those of the women in normal group, and the numbers of enterobacteria, enterococcus, bacteroidetes, and yeasts of the women in group A were significant higher than those of the women in group B (P＜0.05). The serum levels of tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), hypersensitive C-reactive protein (hs-CRP), and CD8+ of the women in group A were significant higher than those of the women in group B, but the levels of CD3+, CD4+ and CD4+/CD8+ of the women in group A were significant lower than those of the women in group B (P＜0.05). Spearman correlation showed that the numbers of enterobacteria, enterococcus, bacteroidetes, and yeast of the women in group A were positively correlated with their levels of TNF-α, IL6, HSCPR, and CD8+ (P＜0.05), and which were negatively correlated with their levels of CD3+, CD4+, and CD4+/CD8+ (P＜0.05). The numbers of lactobacillus and bacteroidetes of the women in group A were negatively correlated with their levels of TNF-α, IL6, HSCPR, and CD8+ (P＜0.05), and which were positively correlated with their levels of CD3+, CD4+, and CD4+/CD8+ (P＜0.05). The total incidence (21.2%) of adverse pregnancy of the women in group A was significant higher than that (6.8%) of the women in group B (P＜0.05). In group A, the numbers of bifidobacteria and lactobacillus colonies of the women with poor pregnancy outcomes were significant less than those of the women with normal pregnancy outcomes, and the numbers of enterobacteria, enterococcus, bacteroidetes, and yeast colonies of the women with poor pregnancy outcomes were significant higher than those of the women with normal pregnancy outcomes (P＜0.05). Conclusion: The pregnant women with GDM has imbalance of intestinal flora, which has certain correlation with their inflammatory factors and T lymphocyte subsets levels, and so has some influence on their pregnancy outcomes.
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