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| Effect of exposure time of frozen semen in room temperature on the freezing recovery rate of forward motile sperm |
| Guangdong Provincial Family planning Research Institute of Science and Technology, Male Reproductive and Genetic Key Laboratory of the National Health Commission, Guangzhou, Guangdong Province, 510600 |
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Abstract To study the effect of repeated exposure time in room temperature of frozen semen on the quality of resuscitated semen. Methods: 40 semen samples from 10 donors in the screening period from human sperm bank of Guangdong family planning hospital in 2018 were divided into 4 groups according to the exposure time of semen in room temperature (26 ℃), which included group A (semen exposed in room temperature 1 minute for 20 times), group B (semen exposed in room temperature 2 minutes for 20 times), group C (semen exposed in room temperature 4 minutes for 20 times), and control group (semen without exposed in room temperature). The percentage of forward motile sperm before and after frozen semen recovery in the four groups was detected. The freezing recovery rate of forward motile sperm in the four groups was calculated. Results: The freezing recovery rate of forward motile sperm in control group was significant higher than that in group B and C (P<0.05), but had no significant different from that in group A (P>0.05). The freezing recovery rate of forward motile sperm in group A was significant higher than that in group B and C (P<0.05). The freezing recovery rate of forward motile sperm in group B was significant higher than that in group C (P<0.05). Conclusion： Repeated exposure in room temperature for more than 2 minutes of frozen semen has significant impact on the freezing recovery rate of forward motile sperm. The longer the exposure time in room temperature of frozen semen, the lower freezing recovery rate of forward motile sperm is. Repeated exposure of frozen semen in room temperature for equal to or less than 1 minute has no significant effect on their freezing recovery rate of
DOI:10.3969/j.issn.1004-8189.2021.06.034 forward motile sperm.
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