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| Clinical of the culture of 4859 ordinary quality embryos or abandoned embryos |
| The First Central Hospital of Tianjin, Tianjin, 300192 |
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Abstract To explore the clinical significance of the culture of abandoned embryos during assisted reproduction and the embryos with insufficient to freeze standard for cryopreserve in fresh embryos transplantation cycle. Methods: The abandoned embryos on the third day (D3) after assisted reproductive therapy and the embryos with insufficient to freeze standard for cryopreserve after transplantation of the infertility women were collected. These embryos were cultured sequentially to the blastocyst stage and then the available blastocysts had been cryopreserved. The relationship between the age, the type of infertility, the method of fertilization, the number of blastomeres of embryos，the number of embryos aggregate cultured, or the grade of the embryo of the women and their blastula formation situation were compared. At the same time, the situations of pregnancy and infants were compared between thawed embryos transplantation and cultured available blastocysts transplantation. Results: There were 4859 abandoned embryos or embryos with insufficient to freeze standard for cryopreserve after fresh embryos transplantation had been collected. And 2020 blastocysts (41.6%) had formed after cultured to the 6th day (D6), which included 661 (32.7%) high quality blastocysts. The formation rate of the embryos with the number of blastomere ≥10 blastocysts and the formation rate of high quality blastocysts on the third day were the highest, followed by those of blastomere 7-9 blastocysts, and the formation rate of the embryos and the formation rate of high quality blastocysts decreased sharply when the number of blastomere ＜6 blastocysts. The blastocyst formation rate and high quality blastocyst formation rate of I-II grade embryo on D3 were significant higher than those of III and IV grade embryos (P<0.05). Collective culture was beneficial to the formation of blastocysts, and the formation rate of high-quality blastocysts was the lowest when 1 embryo was cultured per droplet. There were no significant differences in the rates of clinical pregnancy, abortion, twin birth, and live birth, and birth weight between D3 embryo and blastocyst in the freeze-thaw embryo transfer cycle. In the freeze-thaw embryo transfer cycle, the implantation rate of D3 embryos was significant lower than that of blastocyst. Conclusion: Some ordinary quality embryos and abandoned embryos still have developmental potential. Blastocyst cultured is helpful to screen embryos with high potential development for preserve. Freeze-thaw blastocyst transfer can improve the utilization rate and can save the treatment cost of the infertility women.
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