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| The expression of lncRNA ANRIL and VEGF in villi of women with unexplained recurrent spontaneous abortion |
| The First People's Hospital of Liangshan Prefecture, Sichuan Province, 615000 |
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Abstract To investigate the expression of antisense non-coding RNA in the long non-coding RNA (lncRNA) INK4 locus (ANRIL) and vascular endothelial growth factor (VEGF) in villi of women with unexplained recurrent spontaneous abortion (URSA). Methods: 62 women with URSA were selected in observation group from August 2017 to June 2019, and 62 health women with abortion because of unwanted pregnancy were selected in control group. Fresh placental villi of women in the two groups were collected, and the expression levels of lncRNA ANRIL and VEGF mRNA in villi were detected by real-time fluorescence quantitative PCR (qRT-PCR). Pearson method was used to analyze the correlation between the lncRNA ANRIL level and the VEGF mRNA level in the villi. Multivariate logistic regression analysis was used to analyze the influencing factors of the occurrence of URSA. Results: The expression levels of lncRNA ANRIL (0.74±0.20) and VEGF mRNA (0.30±0.09) in the villi of women in the observation group were all significant lower than those (1.00±0.25, 1.00±0.18) of women in the control group (P＜0.05). LncRNA ANRIL level was significantly positively correlated with VEGF mRNA level (r=0.484, P＜0.05). The lncRNA ANRIL and VEGF mRNA expression levels were protective factors affecting the occurrence of URSA (P＜0.05). Conclusion: The down-regulated of expression levels of lncRNA ANRIL and VEGF in the villi of women with URSA is closely related each other significantly, which may jointly affect the occurrence of URSA.
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