|
|
|
| oninvasive detection of methylation of progesterone receptor B in menstrual blood in patients with endometriosis |
| The Ninth People's Hospital of Suzhou (Wujiang Hospital Affiliated to Nantong University), Suzhou, Jiangsu Province, 215200 |
|
|
|
|
Abstract To investigate the noninvasive detection of methylation of progesterone receptor B (PR-B) status in menstrual effluent of patients with endometriosis. Methods: 40 patients with ovarian endometriosis cyst were selected in study group, and 18 infertility patients who undergone hysteroscopy and laparoscopy examination were included in control group from 2016 to 2017. The endometrium, eutopic endometrium, and the first menstrual effluent after operation of women in the two groups were collected and the methylation status of PR-B was detected by methylation fluorescence PCR. Results: The methylation rate of PR-B promoter region in eutopic endometrium and menstrual effluent of patients in the study group were 55% and 47.5%, which were significant higher than those (22.2% and 16.7%) of patients in the control group (P＜0.05). The methylation status of PR-B in eutopic endometrium and menstrual blood of patients in the study group were positively correlated with the methylation status of PR-B and in endometrium and menstrual effluent of patients in the control group（r=0.558, 0.478, P=0.000, 0.045). Conclusion: Abnormal methylation of PR-B in endometrium maybe involved in the occurrence of endometriosis, and this abnormal methylation can be detected from the endometrial debris in menstrual effluent, so it maybe a new idea for non-invasive diagnosis of endometriosis.
|
|
|
|
|
|
|
|
|
