Abstract To analyze the expression of SEMG1 gene of men with asthenospermia, and to study its effect on the outcome of in vitro fertilization (IVF). Methods: 43 infertility men were selected as the research objects from January 2017 to January 2019. The expression of semg1 gene of spermatozoa of these men were detected by real-time fluorescence quantitative PCR, and the SEMG1 protein expression of spermatozoa and the sperm situation of these men were detected by immunofluorescence technique. The effect of SEMG1 gene expression on the outcome of IVF was explored. Results: SEMG1 gene and protein expressions of sperm of men with moderate and severe asthenospermia were the highest, followed by men with mild asthenospermia, and which of men without asthenospermia was the lowest. As for the outcome of IVF, SEMG1 expression in gene and protein levels of men with <50% sperm was significant higher than that of men with ≥50% sperm. Conclusion: The expression of SEMG1 gene and protein in spermatozoa of men with asthenospermia is higher than that of normal men, and the outcome of IVF of men with asthenospermia is poor, which may be one of the pathological factors to lead to the fertility decrease of men with asthenospermia.
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