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| Expression of zinc alpha-2 glycoprotein and zinc finger and BTB domain containing protein 32 in testicle and peritesticular fat of rats |
| 1.Hebei Chest Hospital, Shijiazhuang，Hebei Province, 050041；2. School of Chemistry & Material Science, Hebei Normal University；3. Key Laboratory for Family Planning & Eugenics of National Commission of Health & Family Planning, Hebei Research Institute of Family Planning；4. Key Laboratory Male Reproductive Health, National Research Institute for Family Planning |
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Abstract To investigate the expression and localization of zinc alpha-2 glycoprotein and zinc finger and BTB domain containing protein 32 (ZBTB32) in testis and peritesticular fat of obese rats. Methods: 20 male SD rats with cleanliness grade were randomly divided into control group (normal feed) and study group (high fat feed). After 12 weeks, the obese rat model was established successfully. The localization of ZAG and ZBTB32 in testis was detected by immunohistochemistry. The mRNA and protein expression of ZAG and ZBTB32 in testis and peritesticular fat of rats were detected by real-time fluorescence quantitative PCR and Western blot. Results: The relative expression of ZAG protein and mRNA in peritesticular fat of rats in the study group were 0.751±0.030 and 1.116±0.021, which were significant lower than those (0.048±0.068 and 1.000±0)) of rats in the control group (P<0.05). The expression of ZAG protein and mRNA in the testis tissue of rats were 0.616±0.025 and 0.820±0.760, which had no significant different from those (0.605±0.033 and 1.000±0) of rats in the control group (P>0.05). The expression of ZBTB32 ZAG protein in the testis tissue and peritesticular fat of rats were 1.514±0.057 and 0.376±0.007, which had no significant different from those (1.477±0.035 and 0.390±0.019) of rats in the control group (P>0.05). The expression of ZBTB32 mRNA in the testis tissue and peritesticular fat of rats were 0.994±0.770 and 0.744±0.650, which had no significant different from those (1.000±0 and 1.000±0) of rats in the control group (P>0.05). Conclusion: The expression of ZAG in peritesticular fat of obese rats decreases significantly, which suggests that obesity may lead to abnormal expression of ZAG and affect the reproduction of male rat.
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