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Abstract To investigate the expression of keratinocyte growth factor (KGF) and receptor (KGFR) of pregnant rats with renal ischemia reperfusion (RIR) induced by postpartum hemorrhage after treated byα-melanocyte stimulating hormone (α-MSH), normal saline, or non intervention. Methods: 60 rats of Sprague Dawley (SD) with full-term pregnancy were selected to establish RIR model, 30 cases of them were injected with normal saline (NS)in group A, the other 30 were injected with α-MSH intraperitoneally in group B. Meanwhile, 30 healthy full-term pregnant rats were selected in group C. At the end of 12 hours after the model completion, the life of these rat in the three groups were ended, and the water content of lung and kidney and renal function of rats were observed and compared among the three groups. Results: The levels of urine volume, serum creatinine (Scr) and urea nitrogen (UN) of rats in group A were significant higher than those of rats in the other two groups, but the urine osmotic pressure of rats in group A was significant lower (P＜0.05). And the urine volume of rats in group B was significant higher than that of rats in group C (P＜0.05), but there were no significant difference in the levels of Scr and BUN between group B and C (P＞0.05). The water content of kidney and lung of rats in group A and B were significant higher than those of rats in group C (P＜0.05). KGF mRNA could be expressed in the kidney and lung tissues of rats in the three groups, but KGF mRNA level of rats in group A was significant lower than that of rats in group B and C (P＜0.05). The average optical density and real-time quantitative of KGFR in kidney and lung tissue of rats in group A were significant lower than that of rats in group B and C (P＜0.05). Conclusion: KGF mRNA and KGFR in the kidney and lung of rats with renal ischemia-reperfusion induced by postpartum hemorrhage are related to their injury. α-MSH can protect theirkidney and lung by affecting their expression level of KGF and KGFR.
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