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| The relationships between the expression of Klotho in placenta of elderly pregnant women with gestational hypertension and their pregnancy outcomes |
| Qingdao Women's and Children's Hospital, Shandong Province, 266034 |
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Abstract Objective: To explore the relationships between the expressions of Klotho in placenta of elderly pregnant women with gestational hypertension and their pregnancy outcomes. Methods: From January 2017 to October 2018, 130 elderly pregnant women with gestational hypertension were selected in this study, which included 78 women equal to or over 35 years old were in the study group and 152 women less than 35 years old were in the control group. The rates of adverse pregnancy outcomes, the incidence of complications during pregnancy, and the situation of perinatal infant in both groups were observed. The level of Klotho mRNA in placenta of all women was detected by qRT-PCR, and western blotting was used to detect Klotho protein level in placenta. ROC curve was used to analyze the value of Klotho protein level for diagnosing adverse pregnancy outcomes. Results: The expression levels of Klotho mRNA and Klotho protein in placenta of women in the study group were 4.56±1.13 and 1.07±0.34, which were significant higher than those of women in the control group (P<0.05). The rates of premature rupture of membranes, placental abruption and preterm labor of women in the study group were 11.5%, 12.8%, and 2.3%, respectively, which were significant higher than those of women in the control group (P<0.05). The incidences of perinatal fetal growth restriction and neonatal death in the study group were 10.3% and 6.4%, which were significant higher than those in the control group (P<0.05). The levels of placental Klotho mRNA and Klotho protein of 32 women with adverse pregnancy outcomes were significant higher than those of women with normal pregnancy outcomes (P<0.05). The area under the curve, the sensitivity, and the specificity of Klotho protein level for diagnosing adverse pregnancy outcomes of elderly women with gestational hypertension was 0.779, 89.1% and 62.5%. Conclusion: The expression of Klotho protein in placenta of the elderly pregnant women with gestational hypertension is related to their pregnancy outcomes, which may predict the adverse pregnancy outcomes early.
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