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| The expressions of serum MiR 126 and VEGF of pregnant women with gestational diabetes mellitus and their correlated with insulin resistance |
| The First People's Hospital of Ziyang, Chongqing, 641300 |
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Abstract Objective: To explore the expressions of serum micro RNA 126 (miR 126) and vascular endothelial growth factor (VEGF) of pregnant women with gestational diabetes mellitus (GDM), and to analyze their correlated with insulin resistance (IR). Methods: 76 pregnant women with GDM who underwent cesarean section were included in GDM group from February 2017 to January 2018, and another 68 normal pregnant women who underwent cesarean section were included in the control group during the same period. The expression of serum miR 126 was detected by qRT PCR, and the level of serum VEGF was detected by enzyme linked immunosorbent assay (ELISA). The levels of fasting blood glucose (FBG), fasting insulin (FINS), glycosylated hemoglobin (HbAlc), total cholesterol (TC), triglyceride (TG), high density lipoprotein (HDL C) and low density lipoprotein (LDL C) levels of women were observed and compared between the two groups. The steady state model was used to calculate the islet resistance index (HOMA IR) for evaluating the degree of insulin resistance. Pearson correlation analysis was used to analyze the relevant of the levels of serum miR 126 and VEGF to the value of HOMA IR of women, and Logistic regression analysis was used to analyze the risk factors of IR of pregnant women with GDM. Results: The levels of serum miR 126, HbAlc, FINS, FPG, TG, LDL C of women in GDM group were significant higher than those of women in the control group, but serum VEGF level and HDL C, and the value of HOMA IR of women in GDM group were significant lower (P<0.05). Pearson correlation analysis showed that level of miR 126 was negative correlation to VEGF level and HOMA IR value (r=0.551, 0.513, P=0.000), while VEGF level was positive correlation to HOMA IR value (r=0.454, P= 0.000). Logistic multivariate analysis showed that levels of serum miR 126 and VEGF were independent risk factors for IR of pregnant women with GDM. Conclusion: The serum levels of miR 126 of pregnant women with GDM decreases, while the serum level of VEGF increases, and miR 126 level was positive correlation to VEGF level. Both of levels of miR 126 and VEGF involve the development of IR of pregnant women with GDM.
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