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| The effect of TLR4 on biological behavior of trophoblast cells in maternal-fetal interface |
| Institute of Reproductive Medicine, Baotou Medical College, Inner Mongolia Autonomous Region, 014040 |
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Abstract Objective: To observe the expression of TLR4 in blastocyst and endometrium befor implantation and to explore its effect on the biological behavior of trophoblast cells. Methods: The expressions of TLR4 protein in mouse blastocyst and endometrium before implantation were shown with immunohistochemistry, and the attachment and outgrowth of blastocyst trophoblast cells were observed by anti-TLR4 antibody with co-culture implantation model of mouse blastocyst and human decidual cells. Results: TLR4 protein was positively expressed in the cytoplasm of the inner cell mass and trophoblast cells of mouse blastocyst. The ratio of attachmen was no significant difference between different concentration of TLR4 antibody group 78.52±2.47、82.3±2.6、77.3±3.0 and control group 87.4±2.3 (P>0.05).The ratio of outgrowth in 100ng/ml group 64.3±4.0 was lower than that of control group 83.5±3.3 (P< 0.05). The ratio of outgrowth in 200ng/ml group 51.3±4.1 and 400ng/ml group 46.2±4.2 was significantly lower than that of control group (P< 0.01). The spreading areas of different concentration groups 63.5±5.0、60.2±5.2、58.5±4.8 was significantly lower than that of control group 194.1±8.2 (P< 0.01), and its expanded area was inhibited in a dosedependent manner. Conclusion: TLR4 does not affect the attachment of blastocyst trophoblast cells, which may be related to the invasion and the further development of embryonic trophoblast cells.
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