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Analysis the influence factors on the proportion of fetal free DNA detection from peripheral blood of pregnant women |
| Anji County Hospital of Traditional Chinese Medicine, Zhejiang Province, 313300 |
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Abstract Objective: To investigate the effect factors on the proportion of fetal free DNA detection from peripheral blood of pregnant women, and to improve the success rate of non-invasive prenatal testing (NIPT). Methods: The data of 1235 pregnant women who had underwent NIPT were retrospectively collected, which included the results of high-throughput sequencing of maternal peripheral blood, the result of high chromosome examination of risk fetus by amniocentesis, and the levels of free beta human chorionic gonadotrophin (HCG) and pregnancy-associated plasma protein A (PAPP-A). Results: The proportion of free DNA detected from women’s peripheral blood was in the range of 4.2%-38.2%, and the average level was 11.2% (SD=5.7%). Linear retrospective analysis showed that the proportion of free DNA was negatively correlated with maternal age and body mass index (r=-0.184 and -0.569, P<0.001), but was positively correlated with the levels of free beta HCG and PAPP-A (r=0.327 and 0.512, P<0.001). The multiple linear regressions also showed that the proportion of free DNA was independently related to above four indicators. In 1235 cases, 31 cases with high risk aneuploidy fetuses were detected, and the proportion of fetuses free DNA in women with true positive was significant higher than that in women with false positive (t=2.337, P=0.018). Conclusion: The success rate of fetal free DNA detection are related to gestational age, BMI and placental protein, so the appropriate detection time should be selected according to maternal characteristics.
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