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| The value of positive rate of high risk HPV mRNA for diagnosing different cervical lesions |
| Jiangbei Branch, Zhongda Hospital Affiliated to Southeast University，Nanjing, Jiangsu Province |
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Abstract Objective: To explore the value of positive rate of high risk HPV mRNA for diagnosing different cervical lesions. Methods: 70 gynecological admissions patients with cervical disease in Zhongda hospital affiliated to southeast university were included in the study, their HPV DNA were detected by gene chip flow-through hybridization, and their high risk HPV mRNA were detected by DNA hybridization capture technology. Cervical histopathology results were as the gold standard, the diagnostic accuracy of HPV DNA and high risk HPV E6 / E7 mRNA for diagnosing cervical lesions were compared. Results: The positive rate of patients with cervical lesions detected by HPV DNA was 68.57%, which slightly higher than that of patients detected by high risk HPV E6 / E7 mRNA (61.43%), but there was no statistically significant different (P>0.05). The positive rates of patients with CINⅠ, Ⅱ and Ⅲ detected by high risk HPV E6/E7 mRNA were significant higher than those of patients with chronic cervicitis (P<0.05). In patients with chronic cervicitis or CIN Ⅰ, the positive rate detected by HPV DNA was significant higher than that detected by high-risk HPV E6 / E7 mRNA (P<0.05). In patients with CIN Ⅱ or CIN Ⅲ, the positive rate detected by high-risk HPV E6 / E7 mRNA had no significant different from that detected by HPV DNA (P>0.05). The consistency of detection by HPV DNA and by HPV mRNA had increased along with the severity of cervical lesions. The sensitivity, specificity and negative predictive value of patients with high-grade cervical lesions detected by HPV mRNA were significant higher than those detected by HPV DNA (P<0.05). Conclusion: The detection of high-risk HPV mRNA has high diagnostic sensitivity and specificity for high-grade cervical lesions, and it has highly accurate for identification of high-low grade cervical lesions.
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