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Abstract Objective: To explore the role of endoplasmic reticulum stress (ER stress) in ovarian cancer cell apoptosis induced by MG132. Methods: SKOV3, A2870 and CAOV3 ovarian cancer cells were divided into control group and different proteasome inhibitor-treated groups; SKOV3 cells were divided randomly into GRP78 small interfering RNA (siRNA) group or random sequence of nucleic acids siRNA group. The three species ovarian cancer cells were treated with proteasome inhibitor MG132(5 nmol/L), PSI（20 nmol/L）or EPOX（10 nmol/L）, respectively. GRP78 mRNA protein expression levels in different cells were analyzed by real time quantitative PCR and Western blot. Cell viability and apoptotic cells were measured by MTT assay and flow cytometry. Results: Proteasome inhibitors increased GRP78 mRNA and protein expression levels. As expression of GRP78 siRNA targeting inducted by GRP78, SKOV3 cell vitality significantly decreased after proteasome inhibitor effection. Conclusion: Inducing GRP78 expression disturbs activity of proteasome inhibitors on resistanting ovarian cancer. Expression of GRP78 might enhance the antitumoral efficacy of proteasome inhibitors on ovarian cancer cells.
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