|
|
|
| Study on apoptosis of ovarian cancer cells induced by proteasome inhibitor via endoplasmic reticulum stress |
|
|
|
|
Abstract Objective: To investigate the role of endoplasmic reticulum (ER) stress in ovarian cancer cell apoptosis induced by MG132. Methods: SKOV3 ovarian cancer cells were divided into control group and different concentrations of MG132-treated groups; also divided into random small interfering RNA (siRNA) and siRNA specific against chop (sichop). The cells were treated with MG132, cell viability and apoptotic cells were evaluated using MTT assay and flow cytometry, respectively. The grp78 and chop mRNA and protein levels were analyzed using real-time reverse transcription polymerase chain reaction and western blot, respectively. Results: The treatment with MG132 decreased cell viability and increased apoptotic rate. MG132 dose-dependently increased grp78 and chop mRNA and protein expression levels in SKOV3 cells. Knockdown chop induction by sichop significantly increased the viability of SKOV3 cells upon MG132 exposure. Conclusion: MG132 induces ER stress in ovarian cancer cells. The chop-mediated apoptotic signal of ER stress might play a critical role in MG132-induced cytotoxicity of ovarian cancer cells.
|
|
|
|
|
|
|
|
|
