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Abstract Objective: To explore the influence of the duration of embryo culture after thawing on clinical pregnancy outcomes in frozen-thawed embryos transfer cycles. Methods: A retrospective analysis was performed on 1061 frozen-thawed embryo transfer cycles, which were divided into three groups according to different cultures times, namely, group A (2-4 hours after thawing, n=96) , group B (＞4-8 hours after thawing, n=615) and group C (＞8 hours after thawing, n=350). The clinical pregnancy rate, embryo implantation rate, abortion rate and live birth rate among groups were compared. Results: There were no significant difference in age, duration of infertility, body mass index, endometrial thickness on the embryo transplant day, the number of embryo transferred among the three groups. There were no significant differences among the three groups in the implantation rate (24.7% vs. 23.4% vs. 28.2%, P>005), clinical pregnancy rate (43.8% vs. 40.8% vs. 44.9%, P>0.05), early abortion rate (4.8% vs. 104% vs. 121%, P>0.05) and live birth rate (40.6% vs. 32.7% vs. 37.4%, P>0.05). Conclusion: The incubation time in vitro of frozen-thawed embryos may not significantly affect the pregnancy outcomes, and the embryo transplant time could be chosen according to clinical and patients' conditions.
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