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| Expressions and clinical significances of intercellular adhesion molecule-1 and hypoxia-inducible factor-1α in the endometrial tissue of infertility patients with endometriosis |
| Yunyang County People's Hospital, Yunyang, Chongqing, 404500 |
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Abstract To analyze the expressions and the clinical significances of intercellular adhesion molecule-1 (ICAM-1) and hypoxia-inducible factor-1α (HIF-1α) in the endometrial tissue of infertility patients with endometriosis (EMs). Methods: A total of 85 infertile patients with EMs who had undergone laparoscopic surgery from April 2021 to April 2023 were selected in observation group, and among them, there were 46 cases with stage Ⅰ-Ⅱ of Ems in group A and 39 cases with stage Ⅲ-Ⅳ of Ems in group B according to the American fertility society staging criteria (r-AFS). In addition, 70 patients with infertility because of simple tubal factor who had undergone hysteroscopy during the same time were selected in control group. The expressions of ICAM-1 mRNA and HIF-1α mRNA in the endometrial tissues of the patients in the two groups were detected by reverse transcription polymerase chain reaction (RT-PCR) method. The expressions of ICAM-1 protein and HIF-1α protein in the endometrial tissues of the patients in the two groups were detected by Western blot method. The serum ICAM-1 and HIF-1α levels of the patients in the two groups were detected by ELISA. Receiver operating characteristic (ROC) curve was plotted to analyze the predictive values of the ICAM-1 and HIF-1α levels of the patients with EMs for their infertility. The correlation between the ICAM-1 level of the infertility patients with EMs and their HIF-1αlevel. Results: The expressions of ICAM-1 and HIF-1αmRNA in the endometrial tissue (2.71±0.22 and 0.87±0.23) of the patients in the observation group were significantly higher than those (0.37±0.12 and 0.47±0.15) of the patients in the control group. The expressions of ICAM-1 and HIF-1α protein (2.37±0.07 and 0.81±0.20) of the patients in the observation group were significantly higher than those (0.10±0.03 and 0.35±0.10) of the patients in the control group. The levels of serum sICAM-1 and HIF-1α (278.42±32.05 ng/ml and 90.95±10.77 ng/ml) of the patients in the observation group were significantly higher than those (194.60±19.55 ng/ml and 11.03±3.34 ng/ml) of the patients in the control group. The serum levels of sICAM-1 and HIF-1α of the patients in group B were significantly higher than those of the patients in group A (all P<0.05). ROC curve analysis showed that the area under the curve, the sensitivity and the specificity of the levels of serum sICAM-1 and HIF-1α of the patients with EMs for predicting their infertility were 0.865, 92.0% and 65.0%, respectively, and which were significantly higher than those of the serum sICAM-1 level or the serum HIF-1α level alone (all P<0.05). Pearson correlation analysis showed that there was no correlation between the ICAM-1mRNA level of the patients and their HIF-1α mRNA level, HIF-1α protein level or serum HIF-1αlevel (all P>0.05). Conclusion: The abnormal expression of ICAM-1 and HIF-1α of the patients with EMs may play an important role in the occurrence and development of their infertility. The levels of serum ICAM-1 and HIF-1α of the infertility patients increase with the severity of their EMs, and which have certain value for predicting the infertility of the patients because of EMs.
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