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| Expressions and the clinical significances of the vascular endothelial growth factor A and hypoxia inducible factor-1αin placental tissue of pregnant women with fetal growth restriction |
| Daqing Oilfield General Hospital, Daqing, Heilongjiang Province, 163001 |
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Abstract To investigate the expressions and clinical significances of the vascular endothelial growth factor A (VEGFA) and hypoxia inducible factor (HIF)-1α in placental tissue of pregnant women with fetal growth restriction (FGR). Methods: From May 2020 to September 2022, 116 pregnant women diagnosed with FGR and hospitalized for delivery in the hospital were selected in study group. Another 116 healthy pregnant women who gave birth in the hospital were included in control group. QRT-PCR was applied to detect the mRNA expressions of VEGFA and HIF-1α in placental tissue of the women in the two groups. Immunohistochemistry was applied to detect the protein expressions of VEGFA and HIF-1α. Pearson correlation was applied to analyze the correlation between the VEGFA level of the women and their HIF-1αlevel and the correlation between the VEGFA and HIF-1αlevels of the women with FGR and their clinical data. Multivariate logistic regression was applied to analyze the factors affecting the FGR occurrence of the women. Results: The positive expression rate (25.9%) of the VEGFA protein of the women in the study group was significantly lower than that (64.7%) of the women in the control group, and the positive expression rate (67.2%) of the HIF-1α protein of the women in the study group was significantly higher than that (24.1%) of the women in the control group. The VEGFA mRNA expression level (0.75±0.20) in the placenta tissue of the women in the study group was significantly lower than that (1.00±0.23) of the women in the control group and the HIF-1αmRNA expression level (1.26±0.25) in the placenta tissue of the women in the study group was significantly higher than that (1.01±0.19) of the women in the control group (all P<0.05). The VEGFA mRNA level in placental tissues of the women with FGR was negative correlation with their HIF-1α mRNA level (P<0.05). The neonatal Apgar score at 1 min after birth, the placental weight, the placental volume and weight of the women with FGR were positively correlated with the expression level of their VEGFA, and were negatively correlated with the expression level of their HIF-1α. The increased HIF1αlevel of the women was a risk factor of their FGR occurrence, and the increased VEGFA level of the women was a protective factor of their FGR occurrence (all P<0.05). Conclusion: The VEGFA expression level in the placental tissue of the pregnant women with FGR is low and their HIF-1α expression level in the placental tissue is high, and both of which of the women affect their FGR occurrence and neonatal birth quality.
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