To study the effects of the human-based management intervention for patients with ovarian cyst surgery in operating room on their postoperative rehabilitation, psychological stress and treatment compliance. Methods: A total of 80 patients who wanted gynecological ovarian cyst surgery were selected and divided into two groups (40 cases in each group) according to random number method from October 2021 to March 2024. The patients in the two groups were given routine nursing for two months, and the patients in the observation group were given human-based management in operating room additionally. The situations of the postoperative rehabilitation, psychological stress, compliance and complications of the patients were compared between the two groups. Results: The time of getting out of bed (16.79±4.12h), the postoperative hospital stay (4.68±0.49d), the catheter indwelling time (8.59±2.27h) and the first exhaust time (26.15±5.35h) of the patients in the observation group were significantly shorter than those (25.64±5.49h, 5.62±0.79d, 13.34±2.84h and 35.24±7.35h) of the patients in the control group. The scores of Hamilton anxiety scale (8.16±2.19 points) and Hamilton depression scale (6.35±1.98 points) at discharge, and the incidence of complications (5.0%) of the patients in the observation group were significantly lower than those (11.63±2.54 points, 9.12±2.12 points and 17.0%) of the patients in the control group. The treatment compliance (97.5%) and the Morisky medication compliance scale score at discharge (6.15±1.24 points) of the patients in the observation group were significantly higher than those (82.5% and 6.15±1.24 points) of the patients in the control group (all P<0.05). Conclusion: Humanbased management intervention for the patients with ovarian cyst surgery in operating room is effective in their postoperative rehabilitation, psychological stress and treatment compliance, and in their reduction of postoperative complications.