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| The expression and the clinical significance of neurodirecting factor 2 in serum, villi tissues and decidual tissues of women with missed abortion |
| Shanxi Provincial Children's Hospital (Shanxi Maternal and Child Health Care Hospital), Taiyuan, Shanxi Province, 030001 |
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Abstract To explore the expression and the clinical significance of the neurodirecting factor 2 (SLIT2) in serum, villi tissues and decidual tissues of women with missed abortion. Methods: The clinical data of 196 women with missed abortion (in study group) admitted to hospital from October 2020 to October 2023 were collected, and 120 women with normal early pregnancy termination were selected in control group during the same period. The serum SLIT2 level of the women in the two groups were detected by ELISA. Real-time fluorescent quantitative polymerase chain reaction was used to detect the expression level of SLIT2 mRNA in villi tissues and decidua tissues of the women in the two groups. Pearson correlation analysis was used to investigate the correlation between the SLIT2 expression level in serum, villi tissues and decidual tissues of the women with missed abortion and their levels of the serum human chorionic gonadotropin (hCG), vascular endothelial growth factor (VEGF) and interleukin-6 (IL-6). Receiver operator characteristic (ROC) curve was used to evaluate the diagnostic value of the SLIT2 level in serum, villi tissues and decidual tissues of the pregnancy women for their missed abortion. Results: The SLIT2 level in serum (6.04±1.12ng/ml), in villi tissues (0.62±0.16 ng/ml) and in decidual tissues (0.45±0.14 ng/ml) of the women in the study group were significantly lower than those (9.15±1.86ng/ml, 1.86±0.33 ng/ml and 1.54±0.27 ng/ml) of the women in the control group. The SLIT2 mRNA expressions of the women in serum, in villi tissues and in decidual tissues of the women in the study group were significantly lower than those of the women in the control group. The levels of hCG (41.16±5.23MIU/mD) and VEGF (91.05±9.26pg/ml) of the women in the study group were significantly lower than those (86.54±8.39MIU/mD and 124.26±12.73pg/ml) of the women in the control group. The level of IL-6 (47.51±5.08pg/ml) of the women in the study group was significantly higher than that (10.37±1.44pg/ml) of the women in the control group. The SLIT2 levels of the women with missed abortion in serum, in villi tissues and in decidual tissues were positively correlated with their levels of serum hCG and VEGF, and were negatively correlated with their serum IL-6 level (all P<0.05). The area under the curve (AUC) of the SLIT2 level of the women in serum, in villi tissues and in decidua tissues for diagnosing their missed abortion were 0.771, 0.903 and 0.865, respectively, the cut-off values of which were 7.59ng/ml, 0.99 ng/ml, and 1.24 ng/ml, respectively, the specificity of which were 57.0%, 87.0%, 66.9%, respectively, and the sensitivity of which were 92.6%, 84.7% and 92.6%, respectively. Conclusion: The SLIT2 levels in serum, in villi tissues and in decidua tissue of the women with missed abortion are decreased, and are related to the inflammation and placental angiogenesis of the women, which is expected to be the potential marker for diagnosing the missed abortion of the women.
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