To investigate the effects of the comprehensive insulation nursing in operating room on the perioperative body temperature, the chills, the postpartum recovery and the newborn health of women after cesarean section. Methods: 76 pregnant women who wanted cesarean section were divided into two groups (38 cases in each group) according to the digital table method from January 7, 2020 to September 21, 2023. The women in the control group were given routine insulation management in operating room, and the women in the research group were given the comprehensive insulation nursing in the operating room. The perioperative period temperature, the time of lactation initiation, the gastrointestinal function recovery time and the time of getting out of bed, the incidence of chills, the newborn health and the maternal nursing satisfaction of the women were compared between the two groups. Results: The body temperature during operation (36.54±0.48℃) and at the end of operation (36.69±0.46℃), the neonatal body temperature (37.02±0.39℃), the neonatal development quotient (87.58±12.11 points) and the maternal nursing satisfaction (92.1%) of the women in the research group were significantly higher than those (35. 64±0.41℃, 36.27±0.24℃, 7%±0.29℃ and 74.16±10.69 points) of the women in the control group. The onset time of lactation (18.23±2.34h), the recovery time of gastrointestinal function (16.09±2.48h), the time of getting out of bed (14.72±1.97h) and the incidence of chills (5.3%) of the women in the research group were significantly less than those (24.69±3.48h, 22.69±3.46h, 20.36±2.64h and 23.7%) of the women in the control group  (all P<0.05). Conclusion: The comprehensive thermal insulation nursing for the women in operating room can effectively maintain their perioperative body temperature, improve their postpartum recovery and newborn health, and which can promote the postpartum recovery of the women.