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| Comparison of the effect of direct smear method and latex immunochromatography method for detecting neisseria gonorrhoeae |
| Jiashan Hospital of Traditional Chinese Medicine, Jiaxing, Zhejiang Province, 314100 |
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Abstract To compare the effect of direct smear method and latex immunochromatography method for detecting neisseria gonorrhoeae. Methods: From August 2017 to August 2022, 216 secretion samples of urethra or cervix uteri of the patients with clinical suspected neisseria gonorrhoeae infection were collected, which included 115 urethra secretion samples of the male patients and 101 cervix uteri secretion samples of the female patients. The bacterial culture method was used as the gold standard, and the results of the latex immunochromatography method and the direct smear method were compared for finding the neisseria gonorrhoeae infection in these samples. Results: The bacterial culture method showed that among 216 secretions samples, there were 173 (80.1%) cases with the positive neisseria gonorrhoeae and 43 (19.9%) cases with the negative neisseria gonorrhoeae. Among 115 male urethral secretions samples, there were 96 (83.5%) cases with the positive neisseria gonorrhoeae and 19 (16.5%) cases with the negative neisseria gonorrhoeae. Among 101 female cervix uteri secretions samples, there were 77 (76.2%) cases were positive for neisseria gonorrhoeae and 24 (23.8%) cases were negative for neisseria gonorrhoeae. Compared to that of the golden standard, the direct smear method showed that 154 (71.3%) cases among 216 samples were positive for neisseria gonorrhoeae, which including 85 (82.6%) cases with the positive neisseria gonorrhoeae among 115 male urethral secretions and 59 (58.4%) cases with the positive neisseria gonorrhoeae among 101 female cervix uteri secretions samples. Compared to that of the golden standard, the latex immunochromatography method showed that 177 (81.9%) cases among 216 samples were positive for neisseria gonorrhoeae, which including 96 (87.8%) cases with the positive neisseria gonorrhoeae among 115 male urethral secretions and 76 (75.3%) cases with the positive neisseria gonorrhoeae among 101 female cervix uteri secretions samples. The sensitivity, the specificity, the positive predictive value, the negative predictive value and the accuracy of the latex immunochromatography method for detecting the neisseria gonorrhoeae in all the secretions samples, in the male urethral secretion samples or in the female cervix uteri secretions samples were significantly higher than those of the direct smear method. The Kappa values between the latex immunochromatography method and the direct smear method for detecting the neisseria gonorrhoeae in all the secretions samples, in the male urethral secretions samples or in the female cervix uteri secretions samples were (0.45±0.07), (0.67±0.10) and (0.29±0.10), respectively, and the Kappa value between the latex immunochromatography method and the direct smear method for detecting the neisseria gonorrhoeae in the male urethral secretions samples was ≥0.6, and which had better consistency. Conclusion: Compared with that of the direct smear method, the latex immunochromatography method has the higher efficiency for detecting the neisseria gonorrhoeae in the secretion samples of the patients with suspected gonorrhea infection. The efficacy of the direct smear method and the latex immunochromatography method for detecting neisseria gonorrhoeae in the secretion samples of the male patients is similar.
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