To study the application effect of the phloroglucinol combined with oxytocin used in vaginal trial delivery of primiparous women with single fetue of full-term pregnancy. Methods: 197 primiparous women with single fetus of full-term pregnancy who wanted vaginal trial delivery were selected and were divided into two groups by random number table method when these women experienced abnormalities in the first stage of labor during the trial delivery period from January 2021 to January 2023. 98 women in the control group were treated with oxytocin, and 99 women in the study group were treated with phloroglucinol combined with oxytocin. The progress of labor, the incidence of cervical edema, the intensity of uterine contractions after medication of the women and the maternal and infant outcomes were compared between two groups. Results: The time from the medication used to entering the active phase of labor (77.3±35.5 min), the duration of the active phase of labor (100.3±51.3 min), and the time from the medication used to the full cervical opening (55.4±25.7 min) of the women in the study group were significantly shorter than those (117.6±51.5 min, 155.3±71.3 and 85.4±43.2 min) of the women in the control group. The proportion of the cervical edema (2.0%) of the women in the study group was significantly lower than that (9.2%) of the women in the control group, and the proportion of the strong uterine contraction (76.8%) of the women in the study group was significantly higher than that (63.3%) of the women in the control group. The natural delivery rate (95.0%) of the women in the study group was significantly higher than that(84.7%) of the women in the control group, and the volume of the blood loss during labor (150.4±55.4ml) and in 6 hours after delivery (250.5±38.3ml) of the women in the study group were significantly lower than those (200.5±44.3ml and 291.4±40.6ml) of the women in the control group (all P＜0.05). There were no significant differences in the proportions of forceps delivery, abnormal fetal position, macrosomia and cephalopalvic disproportion of the women between the two groups (P＞0.05). The proportion of the fetal distress (0) of the women in the study group was significantly lower than that (7.1%) of the women in the control group (P＜0.05). There were no significant differences in the neonatal Apgar scores at 1 min and 5 min after birth and the neonatal weight between the two groups (P＞0.05). Conclusion: The application of phloroglucinol combined with oxytocin used in vaginal trial delivery of primiparous women with single fetus of full-term pregnancy can effectively promote their labor progress, improve their natural delivery rate, and reduce their cesarean section rate and amount of postpartum hemorrhage.