To analyze the role of transversus abdominis plane (TAP) block supported by digital three-dimensional (3D) reconstruction technology for anesthesia during cesarean section and for postoperative analgesia. Methods: 156 pregnant women who wanted cesarean section were selected and were divided into observation and control groups (78 women in each group) according to random principle from December 2020 to May 2022. The women in the control group received ultrasound-guided TAP block, while the women in the observation group received ultrasound-guided TAP precision block supported by 3D reconstruction algorithm. The baseline data, such as the age, the underlying diseases, and the values of preoperative systolic blood pressure, diastolic blood pressure and heart rate, and the clinical indictors, such as the time to the first getting out of bed after anesthesia, the analgesia duration, the scores by visual analogue scale (VAS) at postoperative 2h, 12h, 24h and 48h, and anesthesia-related complications situation of the women in the two groups were recorded and analyzed. Results: The time of getting out of bed for the first time after anesthesia (22.65±5.76h) and the analgesia duration (29.17±3.89h) of the women in the observation group were significantly lower than those (25.56±6.02h and 30.27±4.03h) of the women in the control group (all P＜0.05). The pain degree of the women in the two groups at postoperative 24h was the most significant, and which of the women in the observation group at postoperative 2h and 24h (4.01±0.64 points and 4.12±0.86 points) were significantly lower than those (4.25±0.84 points and 4.42±0.83 points) of the women in the control group (all P＜0.05), and which had no significant difference at postoperative 48h of the women between the two groups (P>0.05). There were no significant differences in the rates of adverse reactions and complications of the women between the two groups (P>0.05). Conclusion: TAP block anesthesia according to the 3D based ultrasound-guided for cesarean section of the women is more intuitive and precise, with better postoperative analgesia, and which can provide the new references for the formulation of the plan with personalized, accurate, safe and effective anesthesia.